[建立大鼠尾胶原凝胶滴包埋培养药物敏感性试验方法]
[Establishing the method of collagen-gel droplet embedded culture drug sensitivity test with rat tail collagen].
作者信息
Hu Ya, Zhou Tao, Liao Quan, Zhang Tai-Ping, Chen Ge, Zhao Yu-Pei
机构信息
Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
出版信息
Zhonghua Wai Ke Za Zhi. 2008 Jul 1;46(13):998-1001.
OBJECTIVE
To explore the clinical application of collagen-gel droplet embedded-culture drug sensitivity test (CD-DST) in the malignant tumors.
METHODS
CD-DST was established with rat tail collagen. Three pancreatic cancer cell lines, surgical resection specimens including 15 cases of pancreatic cancer and 10 cases of gastrointestinal cancer were examined using CD-DST.
RESULTS
The overall achievement ratio of CD-DST for clinical tumor specimens was 80% (20/25). In vitro chemosensitivities of pancreatic carcinoma cells to 5-FU, gemcitabine and oxaliplatin were lower than those of gastrointestinal carcinoma.
CONCLUSIONS
CD-DST with rat tail collagen is a valuable chemosensitivity testing method for malignant tumors. It can be used to realize individualized anticancer chemotherapy.
目的
探讨胶原凝胶微滴包埋培养药敏试验（CD-DST）在恶性肿瘤中的临床应用。
方法
用大鼠尾胶原建立CD-DST。采用CD-DST检测3种胰腺癌细胞系、15例胰腺癌手术切除标本及10例胃肠道癌手术切除标本。
结果
CD-DST对临床肿瘤标本的总体成功率为80%（20/25）。胰腺癌细胞对5-氟尿嘧啶、吉西他滨和奥沙利铂的体外化疗敏感性低于胃肠道癌。
结论
大鼠尾胶原CD-DST是一种有价值的恶性肿瘤化疗敏感性检测方法。可用于实现个体化抗癌化疗。
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