Li Zengxia, Sturm Sonja, Svejda Bernhard, Höger Harald, Schraml Elisabeth, Ingolic Elisabeth, Siegl Veronika, Stuppner Hermann, Pfragner Roswitha
Department of Pathophysiology and Immunology, Center of Molecular Medicine, Medical University of Graz, Graz, Austria.
Anticancer Res. 2008 Sep-Oct;28(5A):2705-13.
Medullary thyroid carcinoma (MTC) is a calcitonin-producing tumor of the thyroid arising from the parafollicular C-cells. MTC is poorly responsive to chemotherapy and radiotherapy, hence the only effective therapy is surgery. Based on this fact, alternative strategies have been sought.
The effects of Cautleya gracilis (Smith) Dandy were investigated for the first time in three human MTC cell lines and in MTC-transplanted mice. Proliferation and viability were quantified by cell counting, WST-1 tests, and ATP luminescent cell viability assays. Apoptosis was studied by DAPI staining, flow cytometry and luminescent assays for caspases 3/7, 8 and 9.
A dose-dependent reduction of proliferation and an induction of apoptosis were found in all MTC cell lines, while normal fibroblasts were not impaired. Similar tumor inhibition was seen in heterotransplanted mice.
Our in vitro and in vivo findings suggest a new potential clinical effect of Cautleya.
甲状腺髓样癌（MTC）是一种起源于甲状腺滤泡旁C细胞的能产生降钙素的肿瘤。MTC对化疗和放疗反应不佳，因此唯一有效的治疗方法是手术。基于这一事实，人们一直在寻找替代策略。
首次在三种人MTC细胞系和MTC移植小鼠中研究了细辛（Smith）丹迪的作用。通过细胞计数、WST-1试验和ATP发光细胞活力测定来量化细胞增殖和活力。通过DAPI染色、流式细胞术和半胱天冬酶3/7、8和9的发光测定来研究细胞凋亡。
在所有MTC细胞系中均发现增殖呈剂量依赖性降低且诱导了细胞凋亡，而正常成纤维细胞未受损害。在异种移植小鼠中也观察到了类似的肿瘤抑制作用。
我们的体外和体内研究结果表明细辛具有新的潜在临床效果。
