Li Yunqing, Wang Yi
Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry, Kunming, People's Republic of China.
Mitochondrial DNA B Resour. 2019 Nov 12;4(2):3961-3962. doi: 10.1080/23802359.2019.1688713.
The first complete chloroplast genome (cpDNA) sequence of was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 164,001 bp in length, contains a large single-copy region (LSC) of 89,271 bp and a small single-copy region (SSC) of 15,984 bp, which were separated by a pair of inverted repeats (IR) regions of 29,373 bp. The genome contains 131 genes, including 85 protein-coding genes, 8 ribosomal RNA genes, and 38 transfer RNA genes. The overall GC content of the whole genome is 36.1% and the corresponding values of the LSC, SSC, and IR regions are 33.8, 29.4, and 41.3%, respectively. Further phylogenomic analysis showed that close to genus in family Zingiberaceae.
本研究通过Illumina HiSeq双端测序数据确定了首个完整的叶绿体基因组(cpDNA)序列。该cpDNA长度为164,001 bp,包含一个89,271 bp的大单拷贝区域(LSC)和一个15,984 bp的小单拷贝区域(SSC),它们被一对29,373 bp的反向重复(IR)区域隔开。该基因组包含131个基因,包括85个蛋白质编码基因、8个核糖体RNA基因和38个转运RNA基因。全基因组的总体GC含量为36.1%,LSC、SSC和IR区域的相应值分别为33.8%、29.4%和41.3%。进一步的系统发育基因组分析表明,其在姜科中与某属关系密切。