Rossi Tiziana, Coppi Andrea, Bruni Elisa, Ruberto Antonio, Giudice Stefania, Baggio Giosué
Department of Biomedical Sciences, Section of Pharmacology, University of Modena and Reggio Emilia, Modena, Italy.
Anticancer Res. 2008 Sep-Oct;28(5A):2765-8.
Mepacrine is an antiproliferative agent, characterised by an aliphatic chain similar to that of natural polyamines whose activation is closely associated with cell proliferation and may lead to malignant transformation and neurodegenerative diseases. This study aims to investigate a possible antagonism between mepacrine and polyamines in tumour proliferation.
MCF-7 and Vero cells were cultured in Eagle's minimum essential medium and then subjected to graded concentrations of putrescine, spermine and spermidine alone and in combination with mepacrine. Methyl thiazole tetrazolium test and Western-blotting were performed.
Putrescine and spermidine at 0.5 mg/l significantly stimulated cell growth, whereas mepacrine treatment confirmed the enhanced p21 expression previously reported by a recent study and growth inhibition. When used in combination, mepacrine antagonized MCF-7 growth induced by polyamines.
Our results suggest that mepacrine may represent a choice in the treatment of tumours induced by the modified concentration of polyamines.
米帕林是一种抗增殖剂，其特征在于具有与天然多胺类似的脂肪链，天然多胺的激活与细胞增殖密切相关，并可能导致恶性转化和神经退行性疾病。本研究旨在探讨米帕林与多胺在肿瘤增殖中可能存在的拮抗作用。
MCF-7细胞和Vero细胞在伊格尔氏最低必需培养基中培养，然后分别用不同浓度的腐胺、精胺和亚精胺单独处理，以及与米帕林联合处理。进行甲基噻唑四氮唑试验和蛋白质印迹分析。
0.5mg/l的腐胺和亚精胺显著刺激细胞生长，而米帕林处理证实了最近一项研究所报道的p21表达增强及生长抑制。联合使用时，米帕林可拮抗多胺诱导的MCF-7细胞生长。
我们的结果表明，米帕林可能是治疗因多胺浓度改变而诱发肿瘤的一种选择。
