Lin Yuh-Tzy, Yang Jai-Sing, Lin Shuw-Yuan, Tan Tzu-Wei, Ho Chin-Chin, Hsia Te-Chun, Chiu Tsan-Hung, Yu Chun-Shu, Lu Hsu-Feng, Weng Yueh-Shan, Chung Jing-Gung
Department of Nursing and Management, Jen-Teh Junior College of Medicine, Miaoli, Taiwan, R.O.C.
Anticancer Res. 2008 Sep-Oct;28(5A):2791-9.
The mechanisms of apoptosis induced by diallyl disulfide (DADS) were explored in human cervical cancer Ca Ski cells. Flow cytometric analysis, DNA gel electrophoresis and DAPI staining demonstrated that DADS induced apoptosis in Ca Ski cells. DADS induced apoptosis through the production of reactive oxygen species and Ca2+, and induced abrogation of mitochondrial membrane potential (Deltapsim) and cleavage of Bid protein (t-Bid). DADS increased the levels of p53, p21 and Bax, but caused a decrease in the level of Bcl-2. DADS also promoted the activities of caspase-3 leading to DNA fragmentation, thus indicating that DADS-induced apoptosis is caspase-3 dependent. In addition, DADS induced an increase in the level of cytochrome c in the cytoplasm, which was released from mitochondria. BAPTA attenuated the Deltapsim abrogation and significantly diminished the occurrence of DADS-induced apoptosis in Ca Ski cells. In conclusion, DADS-induced apoptosis occurs via production of ROS and caspase-3 and a mitochondria-dependent pathway in Ca Ski cells.
在人宫颈癌Ca Ski细胞中探究了二烯丙基二硫化物（DADS）诱导细胞凋亡的机制。流式细胞术分析、DNA凝胶电泳和DAPI染色表明，DADS可诱导Ca Ski细胞凋亡。DADS通过产生活性氧和Ca2+诱导细胞凋亡，并导致线粒体膜电位（Δψm）的消除和Bid蛋白（t-Bid）的裂解。DADS增加了p53、p21和Bax的水平，但导致Bcl-2水平降低。DADS还促进了caspase-3的活性，导致DNA片段化，从而表明DADS诱导的细胞凋亡是caspase-3依赖性的。此外，DADS诱导细胞质中细胞色素c水平升高，细胞色素c从线粒体释放。BAPTA减弱了Δψm的消除，并显著减少了Ca Ski细胞中DADS诱导的细胞凋亡的发生。总之，DADS诱导的细胞凋亡通过活性氧的产生、caspase-3以及Ca Ski细胞中的线粒体依赖性途径发生。
