Ito Yuko, Ichikawa Takafumi, Iwai Tomohisa, Saegusa Yoichi, Ikezawa Tomoaki, Goso Yukinobu, Ishihara Kazuhiko
Department of Regulation Biochemistry, Kitasato University Graduate School of Medical Sciences, Sagamihara, Japan.
J Agric Food Chem. 2008 Dec 24;56(24):12122-6. doi: 10.1021/jf802142n.
Although tea catechins are known to exert a potent antiulcer effect on the alimentary tract, there is scant information concerning their effects on normal mucus cell functions. Using original anti-mucin monoclonal antibodies, we studied the influences of long-term administration of catechins on the quantity and quality of mucin in rat gastrointestinal mucosa. Administration of 0.5% tea catechins significantly increased the mucin content of the ileum, but not the stomach. An enzyme-linked immunosorbent assay (ELISA) showed no remarkable qualitative changes in gastric mucin, but a selective increase and decrease in sulfo- and sialomucins, respectively, in the ileum of rats administered catechins. The ELISA results were consistent with both the immunohistochemical findings and the high-iron diamine-alcian blue staining pattern. These findings indicate that tea catechins modulate ileal mucin metabolism in the ileal mucosa, suggesting that further studies focusing on the ileal epithelium will assist in further elucidation of the mechanism of catechin effects.
尽管已知茶儿茶素对消化道有强大的抗溃疡作用，但关于它们对正常黏液细胞功能的影响却知之甚少。我们使用原始的抗黏蛋白单克隆抗体，研究了长期给予儿茶素对大鼠胃肠道黏膜中黏蛋白数量和质量的影响。给予0.5%的茶儿茶素可显著增加回肠的黏蛋白含量，但对胃没有影响。酶联免疫吸附测定（ELISA）显示胃黏蛋白没有明显的质量变化，但在给予儿茶素的大鼠回肠中，硫黏蛋白和唾液酸黏蛋白分别有选择性的增加和减少。ELISA结果与免疫组织化学结果和高铁二胺-阿尔辛蓝染色模式一致。这些发现表明，茶儿茶素可调节回肠黏膜中的回肠黏蛋白代谢，这表明进一步关注回肠上皮的研究将有助于进一步阐明儿茶素作用的机制。
