Chen Lizhang, Wu Chengqiu, Fan Xuegong, Gao Jianming, Yin Hongling, Wang Tom, Wu Jun, Wen Shi Wu
School of Public Health, Central South University, Changsha, Hunan, China.
Int J Infect Dis. 2009 May;13(3):394-8. doi: 10.1016/j.ijid.2008.08.014. Epub 2008 Nov 25.
To examine the presence of hepatitis B surface antigen (HBsAg), hepatitis B core antigen (HBcAg), and hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) in renal tissues from patients with HBV-related glomerulonephritis.
Renal tissue biopsies taken from patients with HBV-related glomerulonephritis and two control groups were prepared for immunocytochemical detection of HBsAg and HBcAg. HBV cccDNA was examined using a nested PCR.
Of the 63 HBV-related glomerulonephritis patients studied, HBsAg was present in the renal tissues of 48 (76.2%) and HBcAg in the renal tissues of 27 (42.9%). The HBsAg and HBcAg positive rates in HBV-related glomerulonephritis patients were higher than those of the 20 patients with non-HBV-related glomerulonephritis (p<0.05). However, there was no significant difference when the HBV-related glomerulonephritis patients were compared with 12 patients with renal tuberculosis, renal atrophy, renal calculus, and renal tumor with positive serum HBV markers. In patients with HBV-related glomerulonephritis, there was no significant difference in HBsAg and HBcAg positive rates in renal tissue between patients with and without serum hepatitis B e antigen (HBeAg). By nested PCR, two of five patients with HBV-related glomerulonephritis were positive for HBV cccDNA.
The location and replication of HBV in renal tissue make the kidney a potential reservoir for HBV. HBV cccDNA may be key in the search for anti-HBV drugs.
检测乙型肝炎表面抗原（HBsAg）、乙型肝炎核心抗原（HBcAg）以及乙型肝炎病毒（HBV）共价闭合环状DNA（cccDNA）在乙型肝炎病毒相关性肾小球肾炎患者肾组织中的存在情况。
对乙型肝炎病毒相关性肾小球肾炎患者及两个对照组进行肾组织活检，用于HBsAg和HBcAg的免疫细胞化学检测。采用巢式PCR检测HBV cccDNA。
在研究的63例乙型肝炎病毒相关性肾小球肾炎患者中，48例（76.2%）肾组织中存在HBsAg，27例（42.9%）肾组织中存在HBcAg。乙型肝炎病毒相关性肾小球肾炎患者的HBsAg和HBcAg阳性率高于20例非乙型肝炎病毒相关性肾小球肾炎患者（p<0.05）。然而，将乙型肝炎病毒相关性肾小球肾炎患者与12例血清HBV标志物阳性的肾结核、肾萎缩、肾结石及肾肿瘤患者进行比较时，差异无统计学意义。在乙型肝炎病毒相关性肾小球肾炎患者中，血清乙型肝炎e抗原（HBeAg）阳性与阴性患者肾组织中的HBsAg和HBcAg阳性率差异无统计学意义。通过巢式PCR检测，5例乙型肝炎病毒相关性肾小球肾炎患者中有2例HBV cccDNA呈阳性。
HBV在肾组织中的定位和复制使肾脏成为HBV的潜在储存库。HBV cccDNA可能是寻找抗HBV药物的关键。
