肽在固相上的荧光标记

Fluorescent labeling of peptides on solid phase.

作者信息

Katritzky Alan R, Yoshioka Megumi, Narindoshvili Tamari, Chung Alfred, Johnson Jodie V

机构信息

University of Florida, Gainesville, FL 32611-7200, USA.

出版信息

Org Biomol Chem. 2008 Dec 21;6(24):4582-6. doi: 10.1039/b811693h. Epub 2008 Oct 30.

DOI:10.1039/b811693h

PMID:19039367

Abstract

N(alpha)-Fmoc-N(epsilon)-[(7-methoxycoumarin-4-yl)acetyl]-L-lysine (N(alpha)-Fmoc-L-Lys(Mca)-OH) 3 is conveniently prepared by benzotriazole methodology (52% over two steps). N-Acylbenzotriazoles Mca-Bt 2, N(alpha)-Fmoc-L-Lys(Mca)-Bt 4, coumarin-3-ylcarbonyl (Cc)-Bt 5, N(alpha)-Fmoc-L-Lys(Cc)-Bt 7 and N(alpha)-(Cc)-L-Lys(Fmoc)-Bt 9 enable the efficient microwave enhanced solid-phase fluorescent labeling of peptides.

摘要

N(α)-芴甲氧羰基-N(ε)-[(7-甲氧基香豆素-4-基)乙酰基]-L-赖氨酸（N(α)-芴甲氧羰基-L-赖氨酸(Mca)-OH）3可通过苯并三唑方法方便地制备（两步产率为52%）。N-酰基苯并三唑Mca-Bt 2、N(α)-芴甲氧羰基-L-赖氨酸(Mca)-Bt 4、香豆素-3-羰基（Cc）-Bt 5、N(α)-芴甲氧羰基-L-赖氨酸(Cc)-Bt 7和N(α)-(Cc)-L-赖氨酸(Fmoc)-Bt 9能够实现肽的高效微波增强固相荧光标记。