Preza D, Olsen I, Willumsen T, Boches S K, Cotton S L, Grinde B, Paster B J
Institute of Oral Biology, University of Oslo, Norway.
Eur J Clin Microbiol Infect Dis. 2009 May;28(5):509-17. doi: 10.1007/s10096-008-0662-8. Epub 2008 Nov 28.
The present study used a new 16S rRNA-based microarray with probes for over 300 bacterial species to better define the bacterial profiles of healthy root surfaces and root caries (RC) in the elderly. Supragingival plaque was collected from 20 healthy subjects (Controls) and from healthy and carious roots and carious dentin from 21 RC subjects (Patients). Collectively, 179 bacterial species and species groups were detected. A higher bacterial diversity was observed in Controls than in Patients. Lactobacillus casei/paracasei/rhamnosus and Pseudoramibacter alactolyticus were notably associated with most RC samples. Streptococcus mutans was detected more frequently in the infected dentin than in the other samples, but the difference was not significant. Actinomyces was found more frequently in Controls. Thus, species other than Actinomyces and S. mutans may play a role as pathogens of RC. The results from this study were in general agreement with those of our previous study based on 16S rRNA gene sequencing.
本研究使用了一种基于16S rRNA的新型微阵列，其含有针对300多种细菌物种的探针，以更好地确定老年人健康牙根表面和根龋（RC）的细菌谱。从20名健康受试者（对照组）以及21名根龋受试者（患者）的健康和龋坏牙根及龋坏牙本质中收集龈上菌斑。总共检测到179种细菌物种和物种组。观察到对照组的细菌多样性高于患者组。干酪乳杆菌/副干酪乳杆菌/鼠李糖乳杆菌和不解糖假拉米菌与大多数根龋样本显著相关。变形链球菌在感染牙本质中的检出频率高于其他样本，但差异不显著。放线菌在对照组中更常见。因此，除放线菌和变形链球菌外的其他物种可能作为根龋的病原体发挥作用。本研究结果与我们之前基于16S rRNA基因测序的研究结果总体一致。
