van Lohuizen M, Verbeek S, Scheijen B, Wientjens E, van der Gulden H, Berns A
Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam.
Cell. 1991 May 31;65(5):737-52. doi: 10.1016/0092-8674(91)90382-9.
Mo-MLV infection of E mu-myc transgenic mice results in a dramatic acceleration of pre-B cell lymphomagenesis. We have used provirus tagging to identify genes that cooperate with the E mu-myc transgene in B cell transformation. Here we report on the identification of four loci, pim-1, bmi-1, pal-1, and bla-1, which are occupied by proviruses in 35%, 35%, 28%, and 14% of the tumors, respectively. bmi-1, pal-1, and bla-1 represent novel common proviral insertion sites. The bmi-1 gene encodes a 324 amino acid protein with a predominantly nuclear localization. bmi-1 is highly conserved in evolution and contains several motifs frequently found in transcriptional regulators, including a new putative zinc finger motif. No genes have yet been assigned to pal-1 and bla-1. The distribution of proviruses over the four common insertion sites suggests that provirus tagging can be used not only to identify the cooperating oncogenes but also to assign these genes to distinct complementation groups in tumorigenesis.
莫洛尼鼠白血病病毒（Mo-MLV）感染Eμ-myc转基因小鼠会导致前B细胞淋巴瘤发生显著加速。我们利用前病毒标签技术来鉴定在B细胞转化过程中与Eμ-myc转基因协同作用的基因。在此，我们报告鉴定出四个位点，即pim-1、bmi-1、pal-1和bla-1，分别有35%、35%、28%和14%的肿瘤中的前病毒占据这些位点。bmi-1、pal-1和bla-1代表新的常见前病毒插入位点。bmi-1基因编码一种324个氨基酸的蛋白质，主要定位于细胞核。bmi-1在进化过程中高度保守，包含几个在转录调节因子中常见的基序，包括一个新的假定锌指基序。尚未有基因被定位到pal-1和bla-1。前病毒在这四个常见插入位点上的分布表明，前病毒标签技术不仅可用于鉴定协同作用的癌基因，还可将这些基因分配到肿瘤发生过程中不同的互补组中。
