Migliorini P, Corradin G, Corradin S B
Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
J Immunol Methods. 1991 May 17;139(1):107-14. doi: 10.1016/0022-1759(91)90357-l.
Macrophage production of arginine-derived NO2- provides a simple method for detection and quantitation of interferon-gamma (IFN-gamma) in murine cell culture fluids. When the macrophage cell line RAW 264 is cultured overnight with IFN-gamma in the presence of 10 ng/ml LPS, NO2- release, as determined by a simple colorimetric assay, is proportional to the concentration of IFN-gamma and is inhibited by monoclonal antibody to IFN-gamma. A high degree of correlation was obtained when antigen stimulated T lymphocyte supernatants were tested for IFN-gamma by ELISA and the NO2- production assay.
巨噬细胞产生的精氨酸衍生的亚硝酸根为检测和定量小鼠细胞培养液中的干扰素-γ（IFN-γ）提供了一种简单方法。当巨噬细胞系RAW 264在10 ng/ml脂多糖存在的情况下与IFN-γ一起培养过夜时，通过简单的比色测定法测定的亚硝酸根释放量与IFN-γ浓度成正比，并受到抗IFN-γ单克隆抗体的抑制。当通过酶联免疫吸附测定法（ELISA）和亚硝酸根产生测定法检测抗原刺激的T淋巴细胞上清液中的IFN-γ时，获得了高度相关性。
