Rao M, Deshpande V V, Gaikwad S, Mishra C
Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.
Biotechnol Appl Biochem. 1991 Apr;13(2):277-85.
An extracellular laminarinase (1----3)-beta-glucan glucohydrolase (EC 3.2.1.6) was purified from culture filtrates of Penicillium funiculosum. It was homogeneous on polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate. It had a Mr of 14,000 and isoelectric point of pH 4.2. The apparent Km value for lamimarinase was 8.3 mg/ml and Vmax was 8 mumol/min/mg. The distribution of beta-glucosidase activity in two different species of Penicillium showed that P. funiculosum had a higher ratio of extracellular to cell wall bound activity than Penicillium janthinellum. Treatment of mycelia of both species with NaCl, EDTA, Triton X-100, or proteolytic enzymes did not release the cell wall bound beta-glucosidase. Incubation of the mycelia with the laminarinase released 2-4 times more beta-glucosidase than the estimated cell bound activity in P. janthinellum and P. funiculosum.
从绳状青霉的培养滤液中纯化出一种胞外海带多糖酶（1→3）-β-葡聚糖葡萄糖水解酶（EC 3.2.1.6）。在有和没有十二烷基硫酸钠的情况下，它在聚丙烯酰胺凝胶电泳中均呈现均一性。其相对分子质量为14000，等电点为pH 4.2。海带多糖酶的表观米氏常数（Km）值为8.3 mg/ml，最大反应速度（Vmax）为8 μmol/（min·mg）。两种不同青霉属菌种中β-葡萄糖苷酶活性的分布表明，与浅黄青霉相比，绳状青霉胞外活性与细胞壁结合活性的比值更高。用氯化钠、乙二胺四乙酸、曲拉通X-100或蛋白水解酶处理这两种菌种的菌丝体，均未释放出细胞壁结合的β-葡萄糖苷酶。用海带多糖酶孵育菌丝体后，释放出的β-葡萄糖苷酶比浅黄青霉和绳状青霉中估计的细胞结合活性多2至4倍。
