Copa-Patiño J L, Rodríguez J, Reyes F, Pérez-Leblic M I
Departamento de Microbiología y Parasitología, Universidad Alcalá de Henares, Madrid, Spain.
FEMS Microbiol Lett. 1990 Aug;58(3):233-9. doi: 10.1111/j.1574-6968.1990.tb13984.x.
The polysaccharidic effect of a purified 1,3-beta-glucanase, a purified beta-glucosidase, and of partially purified endo-1,3-beta-glucanase from autolysed Penicillium oxalicum cultures on cell wall isolate fractions from the same fungus were studied. Fractionation of 5-day-old cell wall gave rise to a series of fractions that were identified using infrared spectrophotometry. The fractions used were: F1, an alpha-glucan; F3, a beta-glucan; F4, a chitin-glucan; and F4b, a beta-glucan. The fractions were incubated with each of the enzymes and with a mixture of equal parts of the three enzymes and the products of the enzymatic hydrolysis were analyzed after 96 h incubation. The enzymes were found to degrade fraction F4b (beta-glucan); the greatest degree of hydrolysis was reached when the three enzymes were used together, suggesting the need for synergic action by these enzymes in the cell wall degradation process.
研究了纯化的1,3-β-葡聚糖酶、纯化的β-葡萄糖苷酶以及来自自溶草酸青霉培养物的部分纯化的内切-1,3-β-葡聚糖酶对同一真菌细胞壁分离组分的多糖效应。对5日龄的细胞壁进行分级分离，得到一系列组分，并用红外分光光度法进行鉴定。所用的组分有：F1，一种α-葡聚糖；F3，一种β-葡聚糖；F4，一种几丁质-葡聚糖；以及F4b，一种β-葡聚糖。将这些组分分别与每种酶以及三种酶等量混合液一起孵育，孵育96小时后分析酶促水解产物。发现这些酶可降解F4b组分（β-葡聚糖）；三种酶一起使用时水解程度最大，表明这些酶在细胞壁降解过程中需要协同作用。
