Liu Ailin, Chen Wei, Huang Liying, Lin Xinhua
Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, P. R. China.
Chem Pharm Bull (Tokyo). 2008 Dec;56(12):1665-9. doi: 10.1248/cpb.56.1665.
A novel uric acid electrochemical sensor was fabricated by electropolymerization on glassy carbon electrode (GCE) with 4-(2-pyridylazo)-resorcinol (PAR) and its electrochemical property was investigated through cyclic voltammetry. The effect of film thickness on the oxidation response was also studied by electropolymerized scan times. The voltammetric behavior of uric acid (UA) was studied with the poly PAR modified GCE. The modified GCE was used to electrochemically detect the individual of UA and the mixture of UA and ascorbic acid (AA) by cyclic voltammetry (CV) or differential pulse voltammetry (DPV) method. For the ternary mixture containing UA and AA, the two compounds can well be separated from each other at a scan rate of 100 mV s(-1) with a potential difference of 345 mV in DPV between UA and AA. The peak currents of UA oxidation increase linearly with the concentration in ranges of 1.0x10(-8)-5.0x10(-5) mol l(-1), and the detection limits (S/N=3) was 1.0x10(-9) mol l(-1). This method was successfully applied to the determination of uric acid in human urine samples.
通过在玻碳电极（GCE）上用4-（2-吡啶偶氮）间苯二酚（PAR）进行电聚合制备了一种新型尿酸电化学传感器，并通过循环伏安法研究了其电化学性质。还通过电聚合扫描次数研究了膜厚度对氧化响应的影响。用聚PAR修饰的GCE研究了尿酸（UA）的伏安行为。采用修饰的GCE，通过循环伏安法（CV）或差分脉冲伏安法（DPV）对UA个体以及UA和抗坏血酸（AA）的混合物进行电化学检测。对于含有UA和AA的三元混合物，在DPV中，当扫描速率为100 mV s⁻¹时，UA和AA之间的电位差为345 mV，这两种化合物可以很好地相互分离。UA氧化的峰值电流在1.0×10⁻⁸ - 5.0×10⁻⁵ mol l⁻¹范围内随浓度呈线性增加，检测限（S/N = 3）为1.0×10⁻⁹ mol l⁻¹。该方法成功应用于人尿样中尿酸的测定。
