Ellis John A, Allan Gordon, Krakowka Steven
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Am J Vet Res. 2008 Dec;69(12):1608-14. doi: 10.2460/ajvr.69.12.1608.
To determine whether genogroup 1 porcine torque teno virus (g1-TTV) can potentiate clinical disease associated with porcine circovirus type 2 (PCV2).
33 gnotobiotic baby pigs.
Pigs were allocated into 7 groups: group A, 5 uninoculated control pigs from 3 litters; group B, 4 pigs oronasally inoculated with PCV2 alone; group C, 4 pigs inoculated IP with first-passage g1-TTV alone; group D, 4 pigs inoculated IP with fourth-passage g1-TTV alone; group E, 6 pigs inoculated IP with first-passage g1-TTV and then oronasally inoculated with PCV2 7 days later; group F, 6 pigs inoculated IP with fourth-passage g1-TTV and then inoculated oronasally with PCV2 7 days later; and group G, 4 pigs inoculated oro-nasally with PCV2 and then inoculated IP with fourth-passage g1-TTV 7 days later.
6 of 12 pigs inoculated with g1-TTV prior to PCV2 developed acute onset of postweaning multisystemic wasting syndrome (PMWS). None of the pigs inoculated with g1-TTV alone or PCV2 alone or that were challenge exposed to g1-TTV after establishment of infection with PCV2 developed clinical illness. Uninoculated control pigs remained healthy.
These data implicated g1-TTV as another viral infection that facilitates PCV2-induced PMWS. This raises the possibility that torque teno viruses in swine may contribute to disease expression currently associated with only a single infectious agent.
确定1型猪圆环病毒(g1-TTV)是否会加重与2型猪圆环病毒(PCV2)相关的临床疾病。
33只无菌仔猪。
将猪分为7组:A组,来自3窝的5只未接种的对照猪;B组,4只经口接种PCV2的猪;C组,4只腹腔注射第一代g1-TTV的猪;D组,4只腹腔注射第四代g1-TTV的猪;E组,6只腹腔注射第一代g1-TTV,7天后经口接种PCV2的猪;F组,6只腹腔注射第四代g1-TTV,7天后经口接种PCV2的猪;G组,4只经口接种PCV2,7天后腹腔注射第四代g1-TTV的猪。
在PCV2接种前接种g1-TTV的12只猪中有6只出现急性断奶后多系统衰竭综合征(PMWS)。单独接种g1-TTV或PCV2,或在PCV2感染后接触g1-TTV攻击的猪均未出现临床疾病。未接种的对照猪保持健康。
这些数据表明g1-TTV是另一种促进PCV2诱导的PMWS的病毒感染。这增加了猪圆环病毒可能导致目前仅与单一传染源相关的疾病表现的可能性。