Rioux L, Gaudin D P, Bui L K, Grégoire L, DiPaolo T, Bédard P J
Department of Anatomy, Laval University, Québec, Canada.
Neuroscience. 1991;40(1):123-31. doi: 10.1016/0306-4522(91)90179-r.
Female rats were lesioned with 6-hydroxydopamine in the left substantia nigra. At least two weeks later they were tested with amphetamine (5 mg/kg, s.c.) and apomorphine (0.25 mg/kg, s.c.). A cell suspension from the ventral mesencephalon of rat embryos was distributed in three sites in a triangular fashion in the center of the denervated striatum. The amphetamine test was then repeated every month for six months. The pattern of circling to amphetamine before the graft was strictly ipsiversive in all animals. From the first month we observed a progressive change and three patterns of rotation could be observed. In 21% of animals, the total number of ipsiversive turns in 90 min actually increased but during the first 20 min the animals turned contralaterally to the lesion (and to the graft). In 38% of animals, the total number of turns switched from ipsiversive to contraversive with the animals turning initially toward the intact side and during the second half of the test toward the lesion. Finally 41% of rats progressively switched to turning only toward the intact side. In all cases, maximal contraversive turning occurred during the initial 20 min. In these rats, tyrosine hydroxylase-positive cells were detected mainly in the dorsal striatum with a few in the central portion. Moreover there was a strong correlation between the number of surviving grafted neurons and the growth of their fiber into the host striatum and the extent of recovery.(ABSTRACT TRUNCATED AT 250 WORDS)
将雌性大鼠左侧黑质用6-羟基多巴胺损伤。至少两周后，用苯丙胺（5毫克/千克，皮下注射）和阿扑吗啡（0.25毫克/千克，皮下注射）对它们进行测试。将来自大鼠胚胎腹侧中脑的细胞悬液以三角形方式分布在去神经支配纹状体中央的三个部位。然后每月重复一次苯丙胺测试，持续六个月。在移植前，所有动物对苯丙胺的转圈模式严格为向患侧。从第一个月起，我们观察到一种渐进性变化，可观察到三种旋转模式。在21%的动物中，90分钟内患侧转圈的总数实际上增加了，但在最初20分钟内，动物转向与损伤（和移植部位）相反的一侧。在38%的动物中，转圈总数从患侧转向对侧，动物最初转向完整侧，在测试后半段转向损伤侧。最后，41%的大鼠逐渐转变为仅转向完整侧。在所有情况下，最大对侧转圈发生在最初20分钟内。在这些大鼠中，酪氨酸羟化酶阳性细胞主要在背侧纹状体中检测到，中央部分有少数。此外，存活的移植神经元数量与其纤维向宿主纹状体的生长以及恢复程度之间存在很强的相关性。（摘要截取自250字）
