Abrous D N, Torres E M, Annett L E, Reading P J, Dunnett S B
Department of Experimental Psychology, University of Cambridge, UK.
Exp Brain Res. 1992;91(2):181-90. doi: 10.1007/BF00231653.
The aim of the present experiment was to characterize the effect of intrastriatal grafts of embryonic dopaminergic neurones on the expression of Fos protein in the striatum when challenged with amphetamine. Unilateral 6-hydroxydopamine lesions of the nigrostriatal pathway were made in adult rats and grafting was performed 3 weeks later. The numbers of Fos-positive nuclei in the ipsi- and contralateral striata were counted on coronal sections following immunohistochemical staining 5 months after grafting. Administration of d-amphetamine induced an increase in the density of Fos-positive nuclei in the intact striatum. This stimulatory effect of amphetamine on c-fos expression was blocked by 6-hydroxydopamine hydrobromide lesions and was restored in the striata bearing transplants. However, an overshoot was observed as the density of Fos-positive cells within the grafted striatum was larger than that observed within the intact striatum. This hyperexpression of Fos-positive nuclei was correlated with the exaggerated compensation of amphetamine-induced rotation in the same animals.
本实验的目的是在给予苯丙胺刺激时，表征胚胎多巴胺能神经元纹状体内移植对纹状体中Fos蛋白表达的影响。对成年大鼠进行黑质纹状体通路的单侧6-羟基多巴胺损伤，并在3周后进行移植。移植5个月后，通过免疫组织化学染色，在冠状切片上对同侧和对侧纹状体中Fos阳性细胞核的数量进行计数。给予d-苯丙胺可使完整纹状体中Fos阳性细胞核的密度增加。苯丙胺对c-fos表达的这种刺激作用被氢溴酸6-羟基多巴胺损伤所阻断，并在接受移植的纹状体中恢复。然而，观察到一个过冲现象，因为移植纹状体内Fos阳性细胞的密度大于完整纹状体内观察到的密度。Fos阳性细胞核的这种过度表达与同一动物中苯丙胺诱导的旋转的夸张补偿相关。
