Budahn Holger, Peterka Herbert, Mousa Magdi Ali Ahmed, Ding Yunhua, Zhang Shaosong, Li Jinbin
Julius Kühn Institute, Institute for Breeding Research on Horticultural and Fruit Crops, Erwin-Baur-Strasse 27, 06484 Quedlinburg, Germany.
Theor Appl Genet. 2009 Feb;118(4):775-82. doi: 10.1007/s00122-008-0937-6. Epub 2008 Dec 3.
The beet cyst nematode (Heterodera schachtii Schmidt) can be controlled biologically in highly infected soils of sugar beet rotations using resistant varieties of oil radish (Raphanus sativus L. ssp. oleiferus DC.) as a green crop. Resistant plants stimulate infective juveniles to invade roots, but prevent them after their penetration to complete the life cycle. The resistance trait has been transferred successfully to susceptible rapeseed by the addition of a complete radish chromosome. The aim of the study was to construct a genetic map for radish and to develop resistance-associated markers. The map with 545 RAPD, dpRAPD, AFLP and SSR markers had a length of 1,517 cM, a mean distance of 2.8 cM and consisted of nine linkage groups having sizes between 120 and 232 cM. Chromosome-specific markers for the resistance-bearing chromosome d and the other eight radish chromosomes, developed previously from a series of rapeseed-radish addition lines, were enclosed as anchor markers. Each of the extra chromosomes in the addition lines could be unambiguously assigned to one of the radish linkage groups. The QTL analysis of nematode resistance was realized in the intraspecific F(2) mapping population derived from a cross between varieties 'Pegletta' (nematode resistant) x 'Siletta Nova' (susceptible). A dominant major QTL Hs1( Rph ) explaining 46.4% of the phenotypic variability was detected in a proximal position of chromosome d. Radish chromosome-specific anchor markers with known map positions were made available for future recombination experiments to incorporate segments carrying desired genes as Hs1( Rph ) from radish into rapeseed by means of chromosome addition lines.
在甜菜连作的高度感染土壤中，可利用抗线虫的油用萝卜（Raphanus sativus L. ssp. oleiferus DC.）品种作为绿肥作物对甜菜孢囊线虫（Heterodera schachtii Schmidt）进行生物防治。抗性植株会刺激感染性幼虫侵入根部，但在其侵入后阻止它们完成生命周期。通过添加一条完整的萝卜染色体，已成功将抗性性状转移到易感油菜籽中。本研究的目的是构建萝卜的遗传图谱并开发与抗性相关的标记。该图谱包含545个RAPD、dpRAPD、AFLP和SSR标记，长度为1517 cM，平均间距为2.8 cM，由9个连锁群组成，大小在120至232 cM之间。先前从一系列油菜 - 萝卜附加系中开发的、针对携带抗性的d染色体和其他8条萝卜染色体的染色体特异性标记作为锚定标记被纳入其中。附加系中的每条额外染色体都可以明确地分配到萝卜的一个连锁群中。线虫抗性的QTL分析是在品种“Pegletta”（抗线虫）דSiletta Nova”（易感）杂交产生的种内F(2)作图群体中进行的。在d染色体的近端位置检测到一个显性主效QTL Hs1(Rph)，它解释了46.4%的表型变异。具有已知图谱位置的萝卜染色体特异性锚定标记可用于未来的重组实验，以便通过染色体附加系将携带如萝卜中Hs1(Rph)等所需基因的片段整合到油菜籽中。
