Vanasco Virginia, Cimolai Maria Cecilia, Evelson Pablo, Alvarez Silvia
Laboratory of Free Radical Biology, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.
Free Radic Res. 2008 Sep;42(9):815-23. doi: 10.1080/10715760802438709.
The aims of this work were to study the mitochondrial function and to evaluate (a) the oxidative stress in real time in an acute model of endotoxemia and (b) the effect of alpha-lipoic acid (LA, 100 mg/kg) as a therapeutic strategy to be considered. In rats treated with lipopolisaccharide (LPS, 10 mg/kg), a 1.4-fold increase was observed in in situ skeletal muscle chemiluminescence. Experimental sepsis increased oxygen consumption in tissue cubes (1 mm(3)) by 30% for heart and diaphragm and impaired state 3 mitochondrial respiration rate in the three organs (liver, diaphragm and heart) studied. Only complex I activity in heart and diaphragm and complex IV activity in diaphragm were found impaired in this septic model. The production of NO by submitochondrial membranes was found increased by 80% in the diaphragm and by 35% in the heart of septic rats. The treatment with LA prevented the oxidative stress and mitochondrial dysfunction observed in this model.
这项工作的目的是研究线粒体功能，并评估：(a) 在内毒素血症急性模型中实时监测氧化应激，以及 (b) 考虑将α-硫辛酸（LA，100 mg/kg）作为一种治疗策略的效果。在用脂多糖（LPS，10 mg/kg）处理的大鼠中，原位骨骼肌化学发光增加了1.4倍。实验性脓毒症使组织块（1 mm³）中的心脏和膈肌耗氧量增加了30%，并损害了所研究的三个器官（肝脏、膈肌和心脏）的线粒体呼吸状态3速率。在这个脓毒症模型中，仅发现心脏和膈肌中的复合体I活性以及膈肌中的复合体IV活性受损。发现脓毒症大鼠膈肌中的亚线粒体膜产生的NO增加了80%，心脏中增加了35%。LA治疗可预防该模型中观察到的氧化应激和线粒体功能障碍。
