Kondo M
Department of Pharmacology, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
Nihon Yakurigaku Zasshi. 1991 Feb;97(2):85-95. doi: 10.1254/fpj.97.2_85.
Effects of chlorpromazine on the mineralization and alkaline phosphatase activity (ALP) in the tooth germ were examined and compared with those of retinoic acid and HEBP (1-hydroxyethylidene-1, 1-bisphosphonate). Mandibular first molars from 17-day-old mouse embryos were cultured with or without drugs. Calcium content and ALP in the tooth germ increased gradually from 0 to 7 days in culture, the increase of calcium being preceded by that of ALP. Retinoic acid suppressed increases of calcium and ALP in the tooth germ but not in the specimens precultured for 2 days, suggesting that retinoic acid inhibits the mineralization at an early developmental stage of the tooth. HEBP, a physiochemical inhibitor of mineralization, suppressed the increase of calcium, but significantly enhanced the increased of ALP in the tooth germ. Chlorpromazine, which has an antagonistic action towards calmodulin, also suppressed the increases of calcium and ALP in the tooth germ. Calmodulin antagonists W-7 and W-5 similarly suppressed the increases of calcium and ALP; W-5 had less effects on both calcium and ALP. These results indicate that calmodulin may be involved in the regulation of the mineralization in the tooth germ. These drugs are shown to possess different modes of inhibitory action on the mineralization.
研究了氯丙嗪对牙胚矿化和碱性磷酸酶活性（ALP）的影响，并与视黄酸和HEBP（1-羟基亚乙基-1,1-二膦酸）的影响进行了比较。将17日龄小鼠胚胎的下颌第一磨牙在有或无药物的情况下进行培养。在培养的0至7天内，牙胚中的钙含量和ALP逐渐增加，钙的增加先于ALP的增加。视黄酸抑制牙胚中钙和ALP的增加，但对预培养2天的标本没有抑制作用，这表明视黄酸在牙齿发育的早期阶段抑制矿化。HEBP是一种矿化的物理化学抑制剂，它抑制了钙的增加，但显著增强了牙胚中ALP的增加。氯丙嗪对钙调蛋白有拮抗作用，它也抑制了牙胚中钙和ALP的增加。钙调蛋白拮抗剂W-7和W-5同样抑制了钙和ALP的增加；W-5对钙和ALP的影响较小。这些结果表明钙调蛋白可能参与牙胚矿化的调节。这些药物对矿化具有不同的抑制作用模式。
