Matsumoto S, Togari A
Department of Pharmacology, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
Nihon Yakurigaku Zasshi. 1995 May;105(5):331-43. doi: 10.1254/fpj.105.331.
In this study, we introduce a cultivation method for mandibular first molars from mouse embryos. We also investigate the in vitro effects of several drugs on tooth formation. Histological observations demonstrated that the tooth germ dissected from 17-day-old embryonic mice and cultivated for 7 days under the conditions described here showed clear mineralization in the dentin and enamel. Alkaline phosphatase (ALP) activity and calcium content in the tooth germ increased developmentally during 7-day cultivation. The increase of calcium was preceded by that of ALP in the germ. The removal of ALP attached covalently to the external surface of plasma membranes by phosphatidylinositol specific phospholipase C disturbed in vitro mineralization in the tooth germ, suggesting that ALP functioning as an ectoenzyme is involved in the physiological process of tooth formation. To elucidate the effects of calcium regulating hormones and several drugs on tooth mineralization, ALP and calcium content were measured and also the tooth germ was observed histologically. The results obtained from the present study suggest that this in vitro system provides a useful tool for investigating both the direct action of drugs on tooth formation and the mechanisms of drug action.
在本研究中，我们介绍了一种从小鼠胚胎培养下颌第一磨牙的方法。我们还研究了几种药物对牙齿形成的体外作用。组织学观察表明，从17日龄胚胎小鼠分离的牙胚在此处所述条件下培养7天，牙本质和釉质中出现明显矿化。在7天的培养过程中，牙胚中的碱性磷酸酶（ALP）活性和钙含量呈发育性增加。胚中钙的增加先于ALP的增加。用磷脂酰肌醇特异性磷脂酶C去除共价附着在质膜外表面的ALP会干扰牙胚的体外矿化，这表明作为外切酶发挥作用的ALP参与了牙齿形成的生理过程。为了阐明钙调节激素和几种药物对牙齿矿化的影响，测量了ALP和钙含量，并对牙胚进行了组织学观察。本研究获得的结果表明，这种体外系统为研究药物对牙齿形成的直接作用以及药物作用机制提供了一种有用的工具。
