Travers Marie-Agnès, Barbou Annaïck, Le Goïc Nelly, Huchette Sylvain, Paillard Christine, Koken Marcel
France Haliotis, Kerazan, Lilia, Plouguerneau, France.
FEMS Microbiol Lett. 2008 Dec;289(1):34-40. doi: 10.1111/j.1574-6968.2008.01367.x.
Vibrio harveyi is a bacterial marine pathogen that can cause fatal disease in a large range of vertebrates and invertebrates, including the commercially important marine gastropod, Haliotis tuberculata. Since 1997, strains of this bacterium have regularly been causing high mortalities in farmed and wild abalone populations. The way in which the pathogen enters into abalone and the disease transmission mechanisms are thus far unknown. Therefore, a pathogenic strain, ORM4, was green fluorescent protein-tagged and validated both for its growth characteristics and for its virulence as a genuine model for abalone disease. The strain allows V. harveyi quantification by flow cytometry in seawater and in abalone haemolymph as well as the in situ detection of the parasite inside abalone tissues.
哈维氏弧菌是一种海洋细菌病原体，可在包括具有重要商业价值的海洋腹足动物——疣荔枝螺在内的多种脊椎动物和无脊椎动物中引发致命疾病。自1997年以来，该细菌菌株经常在养殖和野生鲍鱼种群中导致高死亡率。迄今为止，病原体进入鲍鱼的方式和疾病传播机制尚不清楚。因此，一株致病菌株ORM4被标记上绿色荧光蛋白，并对其生长特性和作为鲍鱼疾病真实模型的毒力进行了验证。该菌株可通过流式细胞术对海水中和鲍鱼血淋巴中的哈维氏弧菌进行定量分析，还能对鲍鱼组织内的寄生虫进行原位检测。
