Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
J Appl Microbiol. 2009 Jan;106(1):213-20. doi: 10.1111/j.1365-2672.2008.03994.x. Epub 2008 Nov 28.
To assess the potential of essential oils and structurally related synthetic food additives in inhibiting the growth of Clostridium perfringens for the control of necrotic enteritis in chickens.
The antimicrobial activity of essential oils/compounds was measured by determining the inhibition of bacterial growth. Thirty-three of 66 oils/compounds exhibited > or =80% inhibition. Seven with the highest potency were further studied. The oils/compounds had MIC(95) values between 167 and 425 microg ml(-1). Most of them were tolerant to low pH (2.0) and exhibited minor or no inhibition of Lactobacillus isolates from the chicken intestine. When mixed with chicken ileal digesta, the oils/compounds retained their efficacy against C. perfringens, but had little effect on the total number of lactobacilli and anaerobic bacteria in the digesta.
Some essential oils/compounds demonstrated good potential in controlling C. perfringens.
This study has identified candidates of essential oils/compounds for in vivo studies for the control of necrotic enteritis in chickens.
评估精油和结构相关的合成食品添加剂抑制产气荚膜梭菌生长的潜力，以控制鸡坏死性肠炎。
通过测定细菌生长抑制来衡量精油/化合物的抗菌活性。在 66 种油/化合物中有 33 种表现出> = 80%的抑制作用。其中 7 种具有最高效力的进一步研究。油/化合物的 MIC（95）值在 167 和 425μg/ml 之间。它们大多数耐受低 pH（2.0），对来自鸡肠道的乳酸菌分离株的抑制作用较小或没有。当与鸡回肠消化物混合时，油/化合物保持对产气荚膜梭菌的功效，但对消化物中乳酸菌和厌氧菌的总数几乎没有影响。
一些精油/化合物在控制产气荚膜梭菌方面显示出良好的潜力。
本研究鉴定了一些精油/化合物，可用于体内研究控制鸡坏死性肠炎。
