Sourek J, Levin J, Trnka T, Zelenková L
Institute of Hygiene and Epidemiology, Prague, Czechoslovakia.
Vaccine. 1991 Feb;9(2):106-10. doi: 10.1016/0264-410x(91)90265-8.
The lipopolysaccharides, lipopolysaccharide-protein complexes and their lipids A, isolated from Shigella dysenteriae 1, exhibited lethal toxicity (LD50 300-400 micrograms per mouse), pyrogenicity (0.01-1.0 microgram), activity in the Limulus test (10(-3)-10(-12) mg ml-1) and produced a positive local Shwartzman reaction. It was found that these substances bound to Al(OH)3 were capable of successfully protecting mice from challenge with both homologous and heterologous infections (up to 90% of mice survived a challenge with LD100), whereas immunization with substances with no biological carrier exhibited very low or zero protective effects. Furthermore, the positive cross-reactivity of lipids A in the agar immunodiffusion test confirms the wide applicability for vaccination purposes.
从痢疾志贺氏菌1型分离出的脂多糖、脂多糖 - 蛋白质复合物及其脂质A，表现出致死毒性（小鼠半数致死量为300 - 400微克）、致热原性（0.01 - 1.0微克）、鲎试剂试验活性（10(-3)-10(-12)毫克/毫升），并产生阳性局部施瓦茨曼反应。研究发现，这些与氢氧化铝结合的物质能够成功保护小鼠免受同源和异源感染的攻击（高达90%的小鼠在接受100倍半数致死量的攻击后存活），而用没有生物载体的物质进行免疫则表现出非常低或零保护效果。此外，脂质A在琼脂免疫扩散试验中的阳性交叉反应证实了其在疫苗接种方面的广泛适用性。
