Witkowska D, Czarny A, Mulczyk M
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Arch Immunol Ther Exp (Warsz). 1987;35(6):759-62.
Outer membrane proteins (OMP) derived from two antigenically different representatives of Shigella (Sh. flexneri 3a and Sh. sonnei phase I) were tested for the toxicity, pyrogenicity, ability to induce Shwartzman reaction as well as for their influence on the leukocyte system. LD50 dose determined on mice was 28 mg/kg for OMP of Sh. flexneri and 23 mg/kg for OMP of Sh. sonnei. Both preparations injected intravenously to rabbits caused moderate increase of body temperature, expressed by the value 1.8 degrees C. Intravenous administration of protein preparations to rabbits, induced at first leukopenia and then transient leukocytosis. When injected subcutaneously in the dose of 500 micrograms and after 24 h intravenously in the dose 100 micrograms, they produced slight hemorrhagic changes at the site of administration.
对来自两种抗原性不同的志贺氏菌（福氏志贺氏菌3a和宋内氏志贺氏菌I相）的外膜蛋白（OMP）进行了毒性、致热原性、诱导施瓦茨曼反应的能力以及它们对白细胞系统影响的测试。对小鼠测定的半数致死剂量（LD50），福氏志贺氏菌OMP为28毫克/千克，宋内氏志贺氏菌OMP为23毫克/千克。静脉注射到兔子体内的两种制剂均引起体温适度升高，升温值为1.8摄氏度。向兔子静脉注射蛋白质制剂，起初引起白细胞减少，随后是短暂的白细胞增多。当以500微克的剂量皮下注射并在24小时后以100微克的剂量静脉注射时，它们在给药部位产生轻微的出血变化。
