Lu Hongbin, Qin Ling, Lee Kwongman, Cheung Winghoi, Chan Kaiming, Leung Kwoksui
Musculoskeletal Research Laboratory, Department of Orthopaedics & Traumatology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, PR China.
Biochem Biophys Res Commun. 2009 Jan 16;378(3):569-73. doi: 10.1016/j.bbrc.2008.11.074. Epub 2008 Dec 3.
This study was designed to compare the temporal changes of gene expression profile in osteoblastic cell lines (SaOS-2) treated with low-intensity pulsed ultrasound stimulation (LIPUS) using complementary DNA (cDNA) microarrays. SaOS-2 cells were treated with LIPUS for 20min. Thereafter, cells were harvested and RNA was extracted twice at 4 and 24h, respectively. Using cDNA microarrays, 7488 genes with changes in expression in SaOS-2 cells were identified for comparison. Microarray analysis revealed a total of 165 genes in SaOS-2 cells were regulated at 4 and 24h after LIPUS treatment. Except for 30 known LIPUS-regulated genes, our study demonstrated for the first time that over 100 genes were related to the underlying molecular mechanism of LIPUS and suggested that LIPUS might regulate a transient expression of numerous critical genes in osteoblastic cells. These results provide further understanding of the role of LIPUS in the regulation of osteoblastic gene expression potentially involved in the molecular mechanism of osteogenesis in fracture repair.
本研究旨在利用互补DNA（cDNA）微阵列比较低强度脉冲超声刺激（LIPUS）处理的成骨细胞系（SaOS-2）中基因表达谱的时间变化。用LIPUS处理SaOS-2细胞20分钟。此后，分别在4小时和24小时收获细胞并提取RNA两次。使用cDNA微阵列，鉴定出SaOS-2细胞中7488个表达有变化的基因用于比较。微阵列分析显示，LIPUS处理后4小时和24小时，SaOS-2细胞中共有165个基因受到调控。除了30个已知的LIPUS调控基因外，我们的研究首次证明超过100个基因与LIPUS的潜在分子机制有关，并表明LIPUS可能调节成骨细胞中众多关键基因的瞬时表达。这些结果进一步加深了我们对LIPUS在骨折修复中可能参与成骨分子机制的成骨细胞基因表达调控作用的理解。
