Castro Mariana S, Ferreira Tânia Cristina G, Cilli Eduardo M, Crusca Edson, Mendes-Giannini Maria José Soares, Sebben Antonio, Ricart Carlos André O, Sousa Marcelo V, Fontes Wagner
Brazilian Center for Protein Research, Department of Cell Biology, University of Brasilia, CEP 70.910-900, Brasilia/DF, Brazil.
Peptides. 2009 Feb;30(2):291-6. doi: 10.1016/j.peptides.2008.11.003. Epub 2008 Nov 13.
RP-HPLC fractionation of the electrically stimulated skin secretion of the arboreal South American frog Hypsiboas albopunctatus ("spotted treefrog") led to the isolation of a cytolytic C-terminally amidated peptide. This novel peptide, named hylin a1 (Hy-a1), consists of 18 amino acid residues (IFGAILPLALGALKNLIK-NH(2)). The alpha-helical structure of the synthetic hylin a1 peptide was confirmed by CD spectroscopy in the presence of 60% (v/v) TFE. The synthetic peptide displayed broad-spectrum antimicrobial activity against Gram-negative and Gram-positive bacteria including Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis and Pseudomonas aeruginosa and also against fungi (Candida albicans, C. krusei, C. parapsilosis and Cryptococcus neoformans). Hylin a1 was also able to disrupt human erytrocytes (HC(50)=18 microM). Similarity analysis using PSI-BLAST revealed 50-44% of identity to maximins Hv, H16, H15 and H10 from Bombina maxima and also to hylins b1 and b2 (Hy-b1 and Hy-b2) from Hypsiboas lundii (synonym: Hyla biobeba).
对树栖南美蛙Hypsiboas albopunctatus（“斑点树蛙”）电刺激皮肤分泌物进行反相高效液相色谱分离，得到一种具有细胞溶解作用的C端酰胺化肽。这种新型肽名为hylin a1（Hy-a1），由18个氨基酸残基组成（IFGAILPLALGALKNLIK-NH₂）。在60%（v/v）三氟乙醇存在的情况下，通过圆二色光谱法证实了合成的hylin a1肽的α-螺旋结构。该合成肽对革兰氏阴性菌和革兰氏阳性菌具有广谱抗菌活性，包括大肠杆菌、金黄色葡萄球菌、粪肠球菌、枯草芽孢杆菌和铜绿假单胞菌，对真菌（白色念珠菌、克鲁斯念珠菌、近平滑念珠菌和新型隐球菌）也有抗菌活性。Hylin a1还能够破坏人红细胞（半数溶血浓度HC₅₀ = 18 μM）。使用PSI-BLAST进行的相似性分析显示，它与大蹼铃蟾的maximins Hv、H16、H15和H10以及Hypsiboas lundii（同义词：雨蛙属生物贝巴）的hylins b1和b2（Hy-b1和Hy-b2）有50 - 44%的同一性。
