Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy.
Institute of Biostructures and Bioimaging (IBB), National Research Council (CNR), 80131 Naples, Italy.
Int J Mol Sci. 2023 Sep 9;24(18):13888. doi: 10.3390/ijms241813888.
Pandemic and epidemic outbreaks of respiratory viruses are a challenge for public health and social care system worldwide, leading to high mortality and morbidity among the human populations. In light of the limited efficacy of current vaccines and antiviral drugs against respiratory viral infections and the emergence and re-emergence of new viruses, novel broad-spectrum antiviral drugs are needed for the prevention and treatment of these infections. Antimicrobial peptides with an antiviral effect, also known as AVPs, have already been reported as potent inhibitors of viral infections by affecting different stages of the virus lifecycle. In the present study, we analyzed the activity of the AVP Hylin-a1, secreted by the frog , against a wide range of respiratory viruses, including the coronaviruses HCoV-229E and SARS-CoV-2, measles virus, human parainfluenza virus type 3, and influenza virus H1N1. We report a significant inhibitory effect on infectivity in all the enveloped viruses, whereas there was a lack of activity against the naked coxsackievirus B3. Considering the enormous therapeutic potential of Hylin-a1, further experiments are required to elucidate its mechanism of action and to increase its stability by modifying the native sequence.
呼吸道病毒的大流行和爆发对全球公共卫生和社会保健系统构成了挑战，导致人类人口的高死亡率和发病率。鉴于当前疫苗和抗病毒药物对呼吸道病毒感染的疗效有限，以及新病毒的出现和再现，需要新型广谱抗病毒药物来预防和治疗这些感染。具有抗病毒作用的抗菌肽，也称为 AVPs，已被报道为通过影响病毒生命周期的不同阶段来有效抑制病毒感染。在本研究中，我们分析了由青蛙分泌的 AVP Hylin-a1 对多种呼吸道病毒的活性，包括冠状病毒 HCoV-229E 和 SARS-CoV-2、麻疹病毒、人副流感病毒 3 型和流感病毒 H1N1。我们报告说，它对所有包膜病毒的感染性都有显著的抑制作用，而对无衣壳的柯萨奇病毒 B3 则没有活性。考虑到 Hylin-a1 的巨大治疗潜力，需要进一步的实验来阐明其作用机制，并通过修饰天然序列来提高其稳定性。
