Karn Santosh Kumar, Hörchner Franz, Srikitjakarn Lertrak, Baumann Maximillian, Nöckler Karsten
'STOP AI Project, International Development Enterprises (IDE), Bakhundol, Lalitpur, Nepal.
Southeast Asian J Trop Med Public Health. 2008 Sep;39(5):795-9.
This epidemiological cross-sectional study was conducted in five major pig-producing districts of CDR, Nepal from November 2006 to April 2007. A total of 576 slaughtered pigs were randomly selected and diaphragmatic crus muscles (n = 551), corresponding sera (n = 487) and sera only (n = 25) were collected from them. Meat samples were examined by pepsin digestion to evaluate for larvae of Trichinella spp. The sera were investigated using ELISA to evaluate for antibodies against T. spiralis. The doubtful and positive sera from the ELISA test were investigated by end-point ELISA, and the Western blot was used for confirmatory diagnosis. Pepsin digestion did not detect the larvae of Trichinella spp. AB-ELISA showed 2 positive and 14 doubtful results, out of 344 sera analyzed. End-point ELISA and the Western blot had revealed that all 16 samples were truly negative. A questionnaire revealed no rodent control (70%), left over feeding practices (65%), dumping of farm waste (82.5%) and uncooked meat was being used as feed (100%). This study reveals that Trichinella spp has a low prevalence.
2006年11月至2007年4月，在尼泊尔中部发展区的五个主要生猪产区开展了这项流行病学横断面研究。共随机选取576头屠宰猪，采集膈脚肌肉样本（n = 551）、相应血清样本（n = 487）及仅血清样本（n = 25）。采用胃蛋白酶消化法检测肉样中的旋毛虫属幼虫。采用酶联免疫吸附测定（ELISA）法检测血清中抗旋毛虫抗体。对ELISA检测结果可疑及呈阳性的血清进行终点ELISA检测，并采用蛋白质印迹法进行确诊。胃蛋白酶消化法未检测到旋毛虫属幼虫。在344份分析血清中，AB-ELISA检测显示2份阳性和14份可疑结果。终点ELISA检测和蛋白质印迹法显示，所有16份样本均为真阴性。一份调查问卷显示，不存在灭鼠措施（70%）、存在剩料喂养习惯（65%）、存在倾倒农场废弃物现象（82.5%）且存在用生肉作饲料的情况（100%）。本研究表明，旋毛虫属的患病率较低。
