Joshi D D, Moller L N, Maharjan M, Kapel C M O
National Zoonoses and Food Hygiene Research Centre Chagal, Jeevan Smriti Marg, House no. 468/32, Ward no. 13 KMC, GPO BOX 1885, Kathmandu, Nepal.
Vet Parasitol. 2005 Sep 5;132(1-2):155-7. doi: 10.1016/j.vetpar.2005.05.046.
In Nepal, animal husbandry is a major source of income. Pig husbandry is practiced in rural, peri-urban, and urban communities. Free ranging "back yard" pigs and the practice of feeding offal is a very common management practice which potentially allows for the transmission of trichinellosis; however, this zoonosis has never been reported from this region. A total of 425 serum samples were collected from local pigs. These were initially screened by ELISA after which positive samples were examined by Western blot. This procedure identified two samples which had clear specific bands for Trichinella; however, muscle samples tested by HCL-pepsin digestion were found to be negative. If these highly specific serological analyses are confirmed, this would be the first report of trichinellosis in Nepal and a prevention program should be initiated to limit the access of pigs to open garbage dumps which exist both in towns and on farms.
在尼泊尔，畜牧业是主要的收入来源。养猪业在农村、城郊和城市社区都有开展。自由放养的“后院”猪以及用动物内脏喂养的做法是一种非常常见的管理方式，这有可能导致旋毛虫病的传播；然而，该地区从未报告过这种人畜共患病。总共从当地猪身上采集了425份血清样本。这些样本首先通过酶联免疫吸附测定（ELISA）进行筛查，之后对阳性样本进行蛋白质印迹法检测。这一过程鉴定出两份样本具有旋毛虫的清晰特异性条带；然而，经盐酸 - 胃蛋白酶消化检测的肌肉样本结果为阴性。如果这些高度特异性的血清学分析得到证实，这将是尼泊尔旋毛虫病的首次报告，应启动预防计划，限制猪接触城镇和农场中存在的露天垃圾场。
