接受左心室辅助装置支持的心力衰竭患者心肌中调节性微小RNA表达的变化。
Changes in regulatory microRNA expression in myocardium of heart failure patients on left ventricular assist device support.
作者信息
Schipper Marguérite E I, van Kuik Joyce, de Jonge Nicholas, Dullens Hub F J, de Weger Roel A
机构信息
Department of Pathology, University Medical Center Utrecht, Utrecht, The Netherlands.
出版信息
J Heart Lung Transplant. 2008 Dec;27(12):1282-5. doi: 10.1016/j.healun.2008.09.005.
BACKGROUND
Left ventricular assist device support in heart failure patients leads to changes in mRNA and protein expression in the myocardium.
METHODS
MicroRNA's are important regulators of various cellular processes, so changes in their expression were tested by Q-PCR methods.
RESULTS
LVAD support (independently of the duration) leads to a decrease of the expression of miR-1, miR-133a and miR-133b in DCM patients but to an increase in expression in IHD patients. The expression of miR's pre- and post-LVAD in heart failure patients was low compared to the level of miR's in control myocardial tissue.
CONCLUSIONS
Alterations in miRs expression might also be important during repair processes in IHD patients.
背景
心力衰竭患者接受左心室辅助装置支持会导致心肌中mRNA和蛋白质表达发生变化。
方法
微小RNA是各种细胞过程的重要调节因子，因此通过定量聚合酶链反应方法检测其表达变化。
结果
左心室辅助装置支持（与持续时间无关）导致扩张型心肌病患者中miR-1、miR-133a和miR-133b表达降低，但在缺血性心脏病患者中表达增加。与对照心肌组织中微小RNA的水平相比，心力衰竭患者左心室辅助装置植入前后微小RNA的表达较低。
结论
微小RNA表达的改变在缺血性心脏病患者的修复过程中可能也很重要。
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