T4多核苷酸激酶催化的tRNA磷酸化作用。
Phosphorylation of tRNA by T4 polynucleotide kinase.
作者信息
Lillehaug J R, Kleppe K
出版信息
Nucleic Acids Res. 1977 Feb;4(2):373-80. doi: 10.1093/nar/4.2.373.
Abstract
The phosphorylation of various intact tRNA species by T4 polynucleotide kinase has been studied. The apparent Michaelis constant was on the average found to be 100 times lower than for some single-stranded DNAs previously studied. (J.R. Lillehaug and K. Kleppe, (1975) Biochemistry, 14, 1221). Conditions which result in complete phosphorylation of different tRNA species have also been established. Studies on equilibrium constants and the reversibility of the reaction revealed that the phosphorylation reaction is not a true equilibrium reaction under the conditions used in this work.
摘要
对T4多核苷酸激酶使各种完整tRNA物种磷酸化的情况进行了研究。结果发现，表观米氏常数平均比之前研究的某些单链DNA低100倍。（J.R. 利勒豪格和K. 克莱佩，（1975年）《生物化学》，第14卷，第1221页）。还确定了能使不同tRNA物种完全磷酸化的条件。对平衡常数和反应可逆性的研究表明，在本研究使用的条件下，磷酸化反应并非真正的平衡反应。
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