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蛔虫两种主要核糖体DNA大小类别的结构组织

Structural organization of the two main rDNA size classes of Ascaris lumbricoides.

作者信息

Back E, Müller F, Tobler H

出版信息

Nucleic Acids Res. 1984 Feb 10;12(3):1313-32. doi: 10.1093/nar/12.3.1313.

DOI:10.1093/nar/12.3.1313
PMID:6322104
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318579/
Abstract

The two main rDNA size classes in the genome of Ascaris lumbricoides consist of 8.8 kb and 8.4 kb long repeating units present in a quantitative ratio of roughly 10:1. They both contain the genes coding for 18 , 5.8S and 26S ribosomal RNAs. The length heterogeneity is due to a 450 bp long spacer region localized in the longer repeating unit which begins 870 bp upstream of the 5'-end 18S gene. A few additional microheterogeneities in base sequence occur at the 5'-end of the 26S gene. The 18S, 5.8S and 26S coding regions have been mapped on both the 8.8 kb and 8.4 kb repeating units and the localization of the 5'- and 3'-ends of the 18S and 26S genes has been performed by S1 protection. No intervening sequences are present in either coding region of the two main rDNA size classes.

摘要

蛔虫基因组中的两种主要核糖体DNA（rDNA）大小类别由长度分别为8.8 kb和8.4 kb的重复单元组成，其数量比约为10:1。它们都包含编码18S、5.8S和26S核糖体RNA的基因。长度异质性是由于一个450 bp长的间隔区位于较长的重复单元中，该间隔区起始于5'-端18S基因上游870 bp处。在26S基因的5'-端还存在一些碱基序列的微小异质性。18S、5.8S和26S编码区已定位在8.8 kb和8.4 kb的重复单元上，并且通过S1核酸酶保护法确定了18S和26S基因5'-端和3'-端的定位。两种主要rDNA大小类别的任一编码区均不存在间隔序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c4/318579/adcc07715c0b/nar00321-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c4/318579/fb72ead53907/nar00321-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c4/318579/79360b18756a/nar00321-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c4/318579/589da089b206/nar00321-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c4/318579/a12fd967a121/nar00321-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c4/318579/a57a1eef5c44/nar00321-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c4/318579/9618b322afc1/nar00321-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c4/318579/adcc07715c0b/nar00321-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c4/318579/fb72ead53907/nar00321-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c4/318579/79360b18756a/nar00321-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c4/318579/589da089b206/nar00321-0035-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c4/318579/a12fd967a121/nar00321-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c4/318579/a57a1eef5c44/nar00321-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c4/318579/9618b322afc1/nar00321-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c4/318579/adcc07715c0b/nar00321-0041-a.jpg

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