新型合成胶原纤维聚（聚乙醇酸）通过糖蛋白VI刺激血小板聚集。

Novel synthetic collagen fibers, poly(PHG), stimulate platelet aggregation through glycoprotein VI.

作者信息

Inoue Osamu, Suzuki-Inoue Katsue, Shinoda Daisuke, Umeda Yasuto, Uchino Masazumi, Takasaki Sin-ichi, Ozaki Yukio

机构信息

Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Chuo, Yamanashi, Japan.

出版信息

FEBS Lett. 2009 Jan 5;583(1):81-7. doi: 10.1016/j.febslet.2008.11.026. Epub 2008 Dec 4.

DOI:10.1016/j.febslet.2008.11.026

PMID:19059245

Abstract

Novel synthetic collagen fibers, poly(PHG) made by polycondensation of Pro-Hyp-Gly, spontaneously assume polymeric structure with molecular weights greater than 10(5). Its application for biomaterials has been explored, but that for a platelet agonist has not been investigated. Poly(PHG)-induced platelet aggregation independently of thromboxane A(2) and integrin alpha2beta1. Poly(PHG)-induced tyrosine phosphorylation of glycoprotein VI (GPVI)-related molecules and failed to activate GPVI/FcRgamma-deficient platelets. Binding of GPVI to poly(PHG) was confirmed by a surface plasmon resonance spectroscopy, suggesting that poly(PHG) activates platelets through GPVI. Poly(PHG) is an useful research tool to investigate GPVI-mediated signals and a substitute for collagen in platelet functional assays.

摘要

由脯氨酸-羟脯氨酸-甘氨酸缩聚制成的新型合成胶原纤维聚（PHG）能自发形成分子量大于10⁵的聚合物结构。其在生物材料方面的应用已被探索，但作为血小板激动剂的应用尚未被研究。聚（PHG）诱导的血小板聚集独立于血栓素A₂和整合素α2β1。聚（PHG）诱导糖蛋白VI（GPVI）相关分子的酪氨酸磷酸化，但未能激活GPVI/FcRγ缺陷型血小板。表面等离子体共振光谱证实了GPVI与聚（PHG）的结合，表明聚（PHG）通过GPVI激活血小板。聚（PHG）是研究GPVI介导信号的有用研究工具，也是血小板功能测定中胶原蛋白的替代品。