Tian Chang Fu, Wang En Tao, Wu Li Juan, Han Tian Xu, Chen Wen Feng, Gu Chun Tao, Gu Jin Gang, Chen Wen Xin
Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/College of Biological Sciences, China Agricultural University, Beijing, PR China.
Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2871-5. doi: 10.1099/ijs.0.2008/000703-0.
Six strains were isolated from root nodules of Vicia faba grown in Nanchang, Yifeng, Taihu, Huaibei, Bengbu and Lujiang, in the middle and lower reaches of the Yangtze River. According to phylogenetic analyses of 16S rRNA gene, atpD and recA sequences, these strains belong to the genus Rhizobium, with Rhizobium etli and Rhizobium leguminosarum as the closest related species. CCBAU 33202(T), a representative of these novel isolates, showed sequence similarity to its closest relatives R. etli CFN 42(T) and R. leguminosarum USDA 2370(T) of 99.5 and 99.1 % for the 16S rRNA gene, 91.9 and 91.9 % for atpD and 90.3 and 93.2 % for recA. The strains from this study could also be differentiated from R. etli CFN 42(T) and R. leguminosarum USDA 2370(T) by 16S-IGS RFLP and SDS-PAGE of whole-cell proteins, fatty acid profiles and several phenotypic characteristics. DNA-DNA hybridization yielded relatedness of 19 and 14-43 %, respectively, with R. etli CFN 42(T) and strains representing different biovars of R. leguminosarum. All data obtained in this study showed that these V. faba isolates belong to a novel species, for which the name Rhizobium fabae sp. nov. is proposed. The type strain, CCBAU 33202(T) (=LMG 23997(T) =JCM 14381(T)), was isolated from Nanchang.
从长江中下游地区南昌、宜丰、太湖、淮北、蚌埠和庐江种植的蚕豆根瘤中分离出6株菌株。根据16S rRNA基因、atpD和recA序列的系统发育分析，这些菌株属于根瘤菌属，与埃氏根瘤菌和豌豆根瘤菌为亲缘关系最近的物种。这些新分离菌株的代表CCBAU 33202(T)，其16S rRNA基因与亲缘关系最近的埃氏根瘤菌CFN 42(T)和豌豆根瘤菌USDA 2370(T)的序列相似性分别为99.5%和99.1%，atpD为91.9%和91.9%，recA为90.3%和93.2%。本研究中的菌株还可通过16S-IGS RFLP、全细胞蛋白的SDS-PAGE、脂肪酸谱和一些表型特征与埃氏根瘤菌CFN 42(T)和豌豆根瘤菌USDA 2370(T)区分开来。DNA-DNA杂交结果显示，与埃氏根瘤菌CFN 42(T)和代表豌豆根瘤菌不同生物变种的菌株的相关性分别为19%和14 - 43%。本研究获得的所有数据表明，这些蚕豆分离菌株属于一个新物种，为此提出新名称蚕豆根瘤菌（Rhizobium fabae sp. nov.）。模式菌株CCBAU 33202(T)（=LMG 23997(T) =JCM 14381(T)）分离自南昌。
