State Key Laboratories for Agro biotechnology/College of Biological Sciences, China Agricultural University, 100193 Beijing, PR China.
Int J Syst Evol Microbiol. 2009 Dec;59(Pt 12):3051-7. doi: 10.1099/ijs.0.009647-0. Epub 2009 Jul 30.
Isolated from root nodules of Trigonella archiducis-nicolai (Sirj.) Vassilcz. grown in Tibet, China, cells of the bacterial strains CCBAU 85039(T) and CCBAU 85027 were Gram-negative, aerobic, motile, non-spore-forming rods that formed colonies that were semi-translucent and opalescent on yeast extract-mannitol agar. In numerical taxonomy, SDS-PAGE analysis of whole-cell proteins and DNA-DNA hybridization, the two strains were very similar and were different from reference strains of defined Rhizobium species. In the phylogeny based on 16S rRNA gene sequences, they were most similar to Rhizobium etli CFN 42(T) (98.2 % similarity) and R. leguminosarum USDA 2370(T) (97.6 %). Sequence analyses of the housekeeping genes recA, atpD and glnII and the 16S-23S rRNA intergenic spacer, phenotypic characteristics and cellular fatty acid profiles strongly suggested that these two strains represented a novel species within Rhizobium. Cross-nodulation tests and sequencing of nifH and nodA genes showed that these two strains were symbiotic bacteria that nodulated Trigonella archiducis-nicolai, Medicago lupulina, Medicago sativa, Melilotus officinalis, Phaseolus vulgaris and Trigonella foenum-graecum. Based on the results, the novel species Rhizobium tibeticum sp. nov. is described to accommodate the two strains. The type strain is CCBAU 85039(T) (=LMG 24453(T) =CGMCC 1.7071(T)). The DNA G+C content of this strain is 59.7 mol% (T(m)).
从中国西藏种植的 Archiducis-nicolai（Sirj.）Vassilcz 三角草根瘤中分离出的细菌菌株 CCBAU 85039(T) 和 CCBAU 85027 细胞为革兰氏阴性、需氧、能动、非孢子形成的棒状，在酵母提取物-甘露醇琼脂上形成半透明和乳光的菌落。在数值分类学中，全细胞蛋白 SDS-PAGE 分析和 DNA-DNA 杂交表明，这两个菌株非常相似，与定义的根瘤菌种的参考菌株不同。在基于 16S rRNA 基因序列的系统发育中，它们与 Rhizobium etli CFN 42(T)（98.2%相似性）和 R. leguminosarum USDA 2370(T)（97.6%相似性）最为相似。管家基因 recA、atpD 和 glnII 的序列分析以及 16S-23S rRNA 基因间隔区、表型特征和细胞脂肪酸图谱强烈表明，这两个菌株代表 Rhizobium 中的一个新种。交叉结瘤试验和 nifH 和 nodA 基因测序表明，这两个菌株是能够与三角草根瘤结瘤的共生细菌，还可以与 Medicago lupulina、Medicago sativa、Melilotus officinalis、Phaseolus vulgaris 和 Trigonella foenum-graecum 结瘤。基于这些结果，描述了一种新的根瘤菌物种 Rhizobium tibeticum sp. nov. 以容纳这两个菌株。该模式菌株是 CCBAU 85039(T)（=LMG 24453(T) =CGMCC 1.7071(T)）。该菌株的 DNA G+C 含量为 59.7 mol%（Tm）。
