Huo Ying-Yi, Wang Chun-Sheng, Yang Jun-Yi, Wu Min, Xu Xue-Wei
College of Life Sciences, Zhejiang University, Hangzhou, PR China.
Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2885-9. doi: 10.1099/ijs.0.2008/000786-0.
Three Gram-negative, aerobic, motile, halophilic, rod-shaped strains (CN46(T), CN71 and CN74(T)) were isolated from sediment of the East China Sea and subjected to a polyphasic taxonomic study. Strains CN46(T) and CN71 had identical 16S rRNA gene sequences and phenotypic characteristics. Strain CN46(T) was moderately halophilic. Growth of strain CN46(T) was observed between 0.5 and 10.0 % (w/v) NaCl (optimal growth at 3.0-5.0 %) and between pH 6.5 and 9.0. Strain CN74(T) grew over a wider range of pH (pH 6.0-9.5); the optimum NaCl concentration for growth was 1.0-3.0 %. The major fatty acids of strain CN46(T) were C(16 : 1)omega9c, C(16 : 0) and C(12 : 0), whereas strain CN74(T) contained C(16 : 0), C(16 : 1)omega9c, C(18 : 1)omega9c and C(12 : 0). The DNA G+C contents of the three isolates were between 58.0 and 58.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains CN46(T), CN71 and CN74(T) grouped together within the cluster of Marinobacter species. 16S rRNA gene sequence similarities of the three strains with the type strains of Marinobacter species ranged from 94.0 to 97.1 %. The DNA-DNA hybridization values of strain CN74(T) with strains CN46(T) and CN71 were 35.0 and 36.0 %, respectively. Levels of DNA-DNA relatedness between strains CN46(T) and CN74(T) and Marinobacter pelagius CGMCC 1.6775(T), Marinobacter gudaonensis CGMCC 1.6294(T) and Marinobacter koreensis DSM 17924(T) were 15.3-45.2 %. The results of DNA-DNA hybridizations, fatty acid analysis, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the isolates from closely related species. Two novel species are proposed, named Marinobacter mobilis sp. nov. (type strain CN46(T) =CGMCC 1.7059(T) =JCM 15154(T)) and Marinobacter zhejiangensis sp. nov. (type strain CN74(T) =CGMCC 1.7061(T) =JCM 15156(T)).
从中国东海沉积物中分离出三株革兰氏阴性、需氧、运动、嗜盐的杆状菌株（CN46(T)、CN71和CN74(T)），并对其进行了多相分类研究。菌株CN46(T)和CN71具有相同的16S rRNA基因序列和表型特征。菌株CN46(T)为中度嗜盐菌。在0.5%至10.0%（w/v）NaCl（最适生长浓度为3.0 - 5.0%）以及pH 6.5至9.0的条件下可观察到菌株CN46(T)生长。菌株CN74(T)在更宽的pH范围（pH 6.0 - 9.5）内生长；生长的最适NaCl浓度为1.0 - 3.0%。菌株CN46(T)的主要脂肪酸为C(16 : 1)ω9c、C(16 : 0)和C(12 : 0)，而菌株CN74(T)含有C(16 : 0)、C(16 : 1)ω9c、C(18 : 1)ω9c和C(12 : 0)。这三株分离菌的DNA G + C含量在58.0至58.9 mol%之间。基于16S rRNA基因序列的系统发育分析表明，菌株CN46(T)、CN71和CN74(T)在海杆菌属物种簇内聚在一起。这三株菌株与海杆菌属物种模式菌株的16S rRNA基因序列相似性范围为94.0%至97.1%。菌株CN74(T)与菌株CN46(T)和CN71的DNA - DNA杂交值分别为35.0%和36.0%。菌株CN46(T)和CN74(T)与海杆菌属浮游海杆菌CGMCC 1.6775(T)、古岛海杆菌CGMCC 1.6294(T)和韩国海杆菌DSM 17924(T)之间的DNA - DNA相关性水平为15.3 - 45.2%。DNA - DNA杂交、脂肪酸分析以及生理生化测试结果使得这些分离菌能够与密切相关物种进行基因型和表型区分。提出了两个新物种，分别命名为运动海杆菌（Marinobacter mobilis）新种（模式菌株CN46(T) = CGMCC 1.7059(T) = JCM 15154(T)）和浙江海杆菌（Marinobacter zhejiangensis）新种（模式菌株CN74(T) = CGMCC 1.7061(T) = JCM 15156(T)）。
