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Macromolecular crowding in vitro as means of emulating cellular interiors: when less might be more.体外大分子拥挤作为模拟细胞内部环境的手段:少即是多。
Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):E119; author reply E120. doi: 10.1073/pnas.0810077106. Epub 2008 Dec 5.
2
Macromolecular crowding modulates folding mechanism of alpha/beta protein apoflavodoxin.大分子拥挤调节α/β蛋白脱辅基黄素氧还蛋白的折叠机制。
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Protein folding by the effects of macromolecular crowding.大分子拥挤效应下的蛋白质折叠
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Modulation of the Polymerization Kinetics of α/β-Tubulin by Osmolytes and Macromolecular Crowding.渗透溶质和大分子拥挤对α/β-微管蛋白聚合动力学的调节
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Crowding Milleu stabilizes apo-myoglobin against chemical-induced denaturation: Dominance of hardcore repulsions in the heme devoid protein.拥挤环境稳定去辅基肌红蛋白抵抗化学诱导变性:无血红素蛋白中硬核斥力的主导作用。
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Self-Assembly of Pseudo-Isocyanine Chloride as a Sensor for Macromolecular Crowding In Vitro and In Vivo.伪异腈氯的自组装作为体外和体内大分子拥挤的传感器。
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Soft interactions and volume exclusion by polymeric crowders can stabilize or destabilize transient structure in disordered proteins depending on polymer concentration.聚合物拥挤剂引起的软相互作用和体积排斥可根据聚合物浓度使无序蛋白质中的瞬时结构稳定或不稳定。
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Molecular crowding reduces to a similar extent the diffusion of small solutes and macromolecules: measurement by fluorescence correlation spectroscopy.分子拥挤对小分子溶质和大分子扩散的降低程度相似:通过荧光相关光谱法进行测量。
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Macromolecular crowding effects on transcription and translation are regulated by free magnesium ion.大分子拥挤对转录和翻译的影响受游离镁离子的调节。
Biotechnol Appl Biochem. 2020 Jan;67(1):117-122. doi: 10.1002/bab.1827. Epub 2019 Oct 15.
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Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding.利用大分子拥挤效应改进二维和三维皮肤体外模型
J Vis Exp. 2016 Aug 22(114):53642. doi: 10.3791/53642.
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Cell-free protein expression under macromolecular crowding conditions.无细胞蛋白在大分子拥挤环境下的表达。
PLoS One. 2011;6(12):e28707. doi: 10.1371/journal.pone.0028707. Epub 2011 Dec 8.
4
Understanding how the crowded interior of cells stabilizes DNA/DNA and DNA/RNA hybrids-in silico predictions and in vitro evidence.理解细胞拥挤的内部如何稳定 DNA/DNA 和 DNA/RNA 杂种——计算机预测和体外证据。
Nucleic Acids Res. 2010 Jan;38(1):172-81. doi: 10.1093/nar/gkp884. Epub 2009 Oct 23.

本文引用的文献

1
Crowded, cell-like environment induces shape changes in aspherical protein.拥挤的、类似细胞的环境会诱导非球形蛋白质发生形状变化。
Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11754-9. doi: 10.1073/pnas.0803672105. Epub 2008 Aug 12.
2
Emulating a crowded intracellular environment in vitro dramatically improves RT-PCR performance.在体外模拟拥挤的细胞内环境可显著提高逆转录聚合酶链反应(RT-PCR)的性能。
Biochem Biophys Res Commun. 2007 Nov 9;363(1):171-7. doi: 10.1016/j.bbrc.2007.08.156. Epub 2007 Sep 5.
3
Macromolecular crowding: obvious but underappreciated.大分子拥挤现象:显而易见却未得到充分重视。
Trends Biochem Sci. 2001 Oct;26(10):597-604. doi: 10.1016/s0968-0004(01)01938-7.

Macromolecular crowding in vitro as means of emulating cellular interiors: when less might be more.

作者信息

Harve K S, Vigneshwar R, Rajagopalan R, Raghunath M

出版信息

Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):E119; author reply E120. doi: 10.1073/pnas.0810077106. Epub 2008 Dec 5.

DOI:10.1073/pnas.0810077106
PMID:19060184
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2629258/
Abstract
摘要