An Kwang Wook, Lee Ki-Young, Yun Sung Gyu, Choi Cheol Young
Division of Marine Environment & BioScience, Korea Maritime University, Busan, Republic of Korea.
Comp Biochem Physiol B Biochem Mol Biol. 2009 Feb;152(2):177-88. doi: 10.1016/j.cbpb.2008.11.001. Epub 2008 Nov 20.
The cDNAs of three gonadotropin (GTH) subunits (GTHalpha, FSHbeta, and LHbeta) and two GTH receptors (FSHR and LHR) from pituitary and gonads of black porgy were cloned. The nucleotide sequences of the GTHalpha, FSHbeta, and LHbeta cDNA were 354, 363, and 414 base pairs (bps) in length with open reading frames (ORF) encoding peptides of 117, 120, and 137 amino acids, respectively. The FSHR and LHR cDNA was 2118 and 2076 bps in length with ORFs encoding peptides of 705 and 691 amino acids, respectively. To study the mechanism of the estradiol-17beta (E(2)) action, we examined the expression pattern of GTH subunit mRNAs in pituitary and GTH-receptor mRNAs in gonads, and the changes of plasma E(2) level when E(2) treatment was applied to immature black porgy. E(2) treatment increased mRNA expression levels of the genes and plasma E(2) levels, indicating that E(2) stimulated the increases in GTH subunit and GTH-receptor mRNAs. These data indicate that E(2) plays an important regulatory role in the brain-pituitary-gonad axis of immature black porgy. We provide the molecular characterization and expression of the GTH subunits and GTH receptors during sex change in the protandrous black porgy.
克隆了黑鲷垂体和性腺中三种促性腺激素（GTH）亚基（GTHα、FSHβ和LHβ）以及两种GTH受体（FSHR和LHR）的cDNA。GTHα、FSHβ和LHβ cDNA的核苷酸序列长度分别为354、363和414个碱基对（bp），开放阅读框（ORF）分别编码117、120和137个氨基酸的肽段。FSHR和LHR cDNA的长度分别为2118和2076 bp，ORF分别编码705和691个氨基酸的肽段。为了研究雌二醇-17β（E₂）的作用机制，我们检测了垂体中GTH亚基mRNA的表达模式和性腺中GTH受体mRNA的表达模式，以及对未成熟黑鲷进行E₂处理时血浆E₂水平的变化。E₂处理增加了基因的mRNA表达水平和血浆E₂水平，表明E₂刺激了GTH亚基和GTH受体mRNA的增加。这些数据表明，E₂在未成熟黑鲷的脑-垂体-性腺轴中发挥重要的调节作用。我们提供了在雄性先熟的黑鲷性别转换过程中GTH亚基和GTH受体的分子特征及表达情况。
