南方鲶性腺中三种促性腺激素亚基的表达及其在性腺早期发育过程中的可能作用。
Expression of three gonadotropin subunits in Southern catfish gonad and their possible roles during early gonadal development.
作者信息
Wu Fengrui, Zhang Xiuyue, Zhang Weili, Huang Baofeng, Liu Zhihao, Hu Chongjiang, Wang Deshou
机构信息
Key Laboratory of Eco-environments in Three Gorges Reservoir Region Ministry of Education, Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing, China.
出版信息
Comp Biochem Physiol A Mol Integr Physiol. 2009 May;153(1):44-8. doi: 10.1016/j.cbpa.2008.12.013. Epub 2008 Dec 25.
The three gonadotropin (GtH) subunit cDNAs, GtHalpha, FSHbeta and LHbeta, which contain complete open reading frames were isolated from Southern catfish (Silurus meridionalis Chen) ovary. RT-PCR revealed that GtHalpha, FSHbeta and LHbeta mRNA were expressed in ovary, female and male pituitaries, but not in testis. Ontogeny study showed that GtHalpha and FSHbeta expressed in ovary from 25 dah (days after hatching) and LHbeta expressed from 40 dah onwards. The expression levels of these genes in all-female Southern catfish gonad were down-regulated after treatment with tamoxifen from 5 to 25 dah when measured at 65 dah. These results indicated the involvement of the three subunits in gonadal development and sexual differentiation.
从南方鲇（Silurus meridionalis Chen）卵巢中分离出了三个促性腺激素（GtH）亚基cDNA，即GtHα、FSHβ和LHβ，它们包含完整的开放阅读框。逆转录聚合酶链反应（RT-PCR）显示，GtHα、FSHβ和LHβ mRNA在卵巢、雌性和雄性垂体中表达，但在睾丸中不表达。个体发育研究表明，GtHα和FSHβ在孵化后25天（dah）起在卵巢中表达，而LHβ从40 dah开始表达。当在65 dah进行检测时，用他莫昔芬处理5至25 dah的全雌南方鲇性腺后，这些基因在性腺中的表达水平下调。这些结果表明这三个亚基参与了性腺发育和性别分化。
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