Won Sung Wook, Yun Hyung Joong, Yun Yeoung-Sang
Division of Environmental and Chemical Engineering, Research Institute of Industrial Technology, Chonbuk National University, Jeonbuk 561-756, Republic of Korea.
J Colloid Interface Sci. 2009 Mar 1;331(1):83-9. doi: 10.1016/j.jcis.2008.11.005. Epub 2008 Nov 7.
Binding mechanisms of Reactive Orange 16 (RO 16) by the protonated waste biomass of Corynebacterium glutamicum were investigated. The solution pH was found to strongly influence the uptake of RO 16 by C. glutamicum. The biosorption of the dye was reversible at pH <7 but irreversible under basic pH conditions. At acidic pH, the electrostatic interaction was found to be a major binding mechanism. The maximum sorption capacities of the biomass were evaluated to be 156.6+/-6.2 and 64.0+/-2.4 mg/g at pH 2 and 4, respectively. The various forms of RO 16 molecule in aqueous solution were identified by LC/MS to study the hydrolysis of RO 16. FTIR and XPS analyses showed the chemical bonding between biomass surface and dye molecules under basic pH conditions, providing insight to the irreversible binding mechanism.
研究了谷氨酸棒杆菌质子化废弃生物质对活性橙16（RO 16）的结合机制。发现溶液pH对谷氨酸棒杆菌摄取RO 16有强烈影响。在pH <7时，染料的生物吸附是可逆的，但在碱性pH条件下是不可逆的。在酸性pH下，静电相互作用是主要的结合机制。生物质在pH 2和4时的最大吸附容量分别评估为156.6±6.2和64.0±2.4 mg/g。通过LC/MS鉴定了水溶液中RO 16分子的各种形式，以研究RO 16的水解。FTIR和XPS分析表明在碱性pH条件下生物质表面与染料分子之间存在化学键合，这为不可逆结合机制提供了见解。
