Chen Ze, Yu Zhijun, Yang Xiaojun, Zheng Hongyuan, Liu Jingze
College of Life Sciences, Hebei Normal University, No. 113, Yuhua Road, Shijiazhuang 050016, China.
Vet Parasitol. 2009 Mar 9;160(1-2):134-7. doi: 10.1016/j.vetpar.2008.10.028. Epub 2008 Oct 19.
The developmental stages in the life cycle of Hyalomma asiaticum kozlovi were investigated under laboratory conditions. The larval, nymphal and adult ticks were all fed on rabbits at 25-27 degrees C, 50% relative humidity (RH) and exposed to daylight. All free-living stages were maintained in an incubator at 26+/-1 degrees C, 70% RH and daylight conditions. The life cycle of H. asiaticum kozlovi was completed in an average period of 151.6 days (range 104-190). The average developmental periods were 38.8 days for egg incubation, 2.1, 5.8 and 10.7 days for larval prefeeding, feeding and premoulting, and 3.3, 6.0 and 26.0 days for the same stages of nymphs, respectively. The average times for prefeeding, feeding, preoviposition and oviposition of female adult ticks were 1.4, 10.1, 20.9, and 26.5 days, respectively. The results confirmed the significant correlation between the weight of the engorged female and the amount of the egg mass laid (r=0.9641, p<0.05). The reproductive efficiency index (REI) and reproductive fitness index (RFI) in females were 8.82 and 7.56, respectively. Engorged nymphs moulting to females were significantly heavier (33.3+/-0.65 mg) than those moulting to males (27.1+/-0.79 mg). The overall sex ratio of the adult ticks was 1:1.07 (M:F).
在实验室条件下研究了亚洲璃眼蜱（Hyalomma asiaticum kozlovi）生活史中的发育阶段。幼虫、若虫和成虫均在25 - 27摄氏度、相对湿度50%（RH）的条件下以兔子为食，并暴露于日光下。所有自由生活阶段均饲养在温度为26±1摄氏度、相对湿度70%及日光条件的培养箱中。亚洲璃眼蜱的生活史平均历时151.6天（范围为104 - 190天）。卵孵化的平均发育时间为38.8天，幼虫取食前、取食和蜕皮前的平均发育时间分别为2.1天、5.8天和10.7天，若虫相同阶段的平均发育时间分别为3.3天、6.0天和26.0天。成年雌蜱取食前、取食、产卵前和产卵的平均时间分别为1.4天、10.1天、20.9天和26.5天。结果证实饱血雌蜱体重与产卵量之间存在显著相关性（r = 0.9641，p < 0.05）。雌蜱的繁殖效率指数（REI）和繁殖适合度指数（RFI）分别为8.82和7.56。蜕皮为雌虫的饱血若虫（33.3±0.65毫克）显著重于蜕皮为雄虫的饱血若虫（27.1±0.79毫克）。成年蜱的总体性别比为1:1.07（雄:雌）。
