Kuzuya M, Naito M, Funaki C, Hayashi T, Yamada K, Asai K, Kuzuya F
Department of Geriatrics, Nagoya University School of Medicine, Japan.
Artery. 1991;18(3):115-24.
The effect of antioxidants on the proliferation of cultured endothelial cells (ECs) were studied. Probucol, a lipid-lowering drug with antioxidant properties, added to the growth medium stimulated the proliferation of ECs. D1-alpha-tocopherol (tocopherol), a natural antioxidant, and butylated hydroxytoluene, a synthetic antioxidant also had the same effect on the proliferation of ECs. There was no significant difference in the content of thiobarbituric acid-reacting substances between the probucol-containing growth medium and control medium during the growth assay. Pretreatment of ECs with probucol or tocopherol also enhanced ECs proliferation in medium without any added antioxidants. The addition of free radical scavengers superoxide dismutase, catalase and mannitol to the growth medium did not enhance the proliferation of ECs. These findings indicate that the stimulatory effect on ECs proliferation of antioxidants in the growth medium appears to be unrelated to preventing free radical reactions in the medium itself, but rather may be related to the uptake of antioxidants by ECs.
研究了抗氧化剂对培养的内皮细胞（ECs）增殖的影响。普罗布考是一种具有抗氧化特性的降脂药物，添加到生长培养基中可刺激ECs的增殖。D1-α-生育酚（生育酚），一种天然抗氧化剂，以及丁基羟基甲苯，一种合成抗氧化剂，对ECs的增殖也有相同的作用。在生长试验期间，含普罗布考的生长培养基和对照培养基之间硫代巴比妥酸反应物质的含量没有显著差异。用普罗布考或生育酚预处理ECs也可增强无任何添加抗氧化剂的培养基中ECs的增殖。向生长培养基中添加自由基清除剂超氧化物歧化酶、过氧化氢酶和甘露醇并不能增强ECs的增殖。这些发现表明，生长培养基中抗氧化剂对ECs增殖的刺激作用似乎与防止培养基本身的自由基反应无关，而可能与ECs对抗氧化剂的摄取有关。
