Kakizaki Y, Kraft N, Atkins R C
Department of Nephrology, Prince Henry's Hospital, Melbourne, Australia.
Clin Exp Immunol. 1991 Jul;85(1):157-63. doi: 10.1111/j.1365-2249.1991.tb05697.x.
Rat mesangial cells were shown to be sensitive to recombinant interferon-gamma (IFN-gamma). IFN-gamma reduced thymidine uptake by these cells and inhibited cell proliferation. Incubation of the cells with 1000 U/ml IFN-gamma decreased thymidine uptake by up to 64% and cell numbers were decreased by 17%. The effects of IFN-gamma were dose and time dependent and were partially reversible by the anti-IFN-gamma monoclonal antibody DB-1. This lymphokine did not reduce incorporation of RNA and protein precursors however. Measurements of 3H-uridine and 3H-leucine incorporation indicated significant increases in RNA and protein synthesis (37% and 45%, respectively) on a per cell basis. The mitogenic effects of IL-1 and platelet-derived growth factor (PDGF) were also susceptible to IFN-gamma-mediated inhibition but the mitogenic response to epidermal growth factor (EGF) was much less sensitive. We conclude that while IFN-gamma may act to modulate the mitogenic signals provided by some factors such as IL-1 and PDGF, the response to EGF appears to be unaffected.
大鼠系膜细胞被证明对重组干扰素-γ（IFN-γ）敏感。IFN-γ减少了这些细胞对胸苷的摄取并抑制细胞增殖。用1000 U/ml IFN-γ孵育细胞，胸苷摄取最多可减少64%，细胞数量减少17%。IFN-γ的作用呈剂量和时间依赖性，且可被抗IFN-γ单克隆抗体DB-1部分逆转。然而，这种淋巴因子并未减少RNA和蛋白质前体的掺入。对3H-尿苷和3H-亮氨酸掺入的测量表明，以每个细胞为基础，RNA和蛋白质合成显著增加（分别为37%和45%）。白细胞介素-1（IL-1）和血小板衍生生长因子（PDGF）的促有丝分裂作用也易受IFN-γ介导的抑制，但对表皮生长因子（EGF）的促有丝分裂反应敏感性要低得多。我们得出结论，虽然IFN-γ可能起到调节某些因子（如IL-1和PDGF）提供的促有丝分裂信号的作用，但对EGF的反应似乎未受影响。
