Lovett D H, Ryan J L, Sterzel R B
J Immunol. 1983 Dec;131(6):2830-6.
Conditioned media from LPS-activated rat peritoneal macrophages enhanced the proliferation rates of cultured rat glomerular mesangial cells. This macrophage-derived activity extensively co-purified with interleukin 1 (IL 1) activity through sequential ammonium sulfate precipitation, S-200 gel chromatography, DEAE-cellulose anion exchange chromatography, and phenyl-Sepharose chromatography. In addition, the macrophage-derived factor was heat-labile (80 degrees C) and inactivated by phenylglyoxal, thus allowing tentative identification as IL 1. Macrophage supernatants and purified IL 1 enhanced the proliferative rates of mesangial cells only in the presence of serum; the use of platelet-poor plasma or serum depleted of platelet-derived growth factor was without effect. IL 1 acted to increase the percentage of cycling cells, without a change in the length of the individual cell cycle times. These findings provide a potential mechanism whereby activated macrophages, in combination with platelet factors, enhance mesangial cell proliferation. Such processes may contribute to the mesangial hypercellularity frequently found in immune-mediated glomerulonephritis.
来自脂多糖激活的大鼠腹膜巨噬细胞的条件培养基可提高培养的大鼠肾小球系膜细胞的增殖率。这种巨噬细胞衍生的活性物质通过连续的硫酸铵沉淀、S-200凝胶色谱、DEAE-纤维素阴离子交换色谱和苯基琼脂糖色谱与白细胞介素1（IL-1）活性广泛共纯化。此外，巨噬细胞衍生因子对热不稳定（80℃），并被苯乙二醛灭活，因此初步鉴定为IL-1。巨噬细胞上清液和纯化的IL-1仅在有血清存在时才提高系膜细胞的增殖率；使用贫血小板血浆或去除血小板衍生生长因子的血清则无作用。IL-1的作用是增加细胞周期细胞的百分比，而单个细胞周期时间的长度没有变化。这些发现提供了一种潜在机制，即活化的巨噬细胞与血小板因子共同作用可增强系膜细胞增殖。此类过程可能导致免疫介导的肾小球肾炎中常见的系膜细胞增多。
