Macías-Sánchez M D, Mantell C, Rodríguez M, Martínez de la Ossa E, Lubián L M, Montero O
Department of Chemical Engineering, Food Technology and Environmental Technologies, Science Faculty, University of Cadiz, Puerto Real, Cádiz, Spain.
Talanta. 2009 Jan 15;77(3):948-52. doi: 10.1016/j.talanta.2008.07.032. Epub 2008 Jul 25.
In the work described here the extraction processes of carotenoids and chlorophylls were analysed using two extraction techniques, namely ultrasound-assisted extraction and supercritical fluid extraction, and the results are compared. The solvents used for the ultrasound-assisted extraction were N,N'-dimethylformamide and methanol and for the supercritical fluid extraction, carbon dioxide. The raw material studied was Dunaliella salina, a microalgae characterized by the high levels of carotenoids present in its cellular structure. The results indicate that the supercritical fluid extraction process is comparable to the ultrasound-assisted extraction when methanol is used as solvent. In addition, the supercritical extraction process is more selective for the recovery of carotenoids than the conventional technique since it leads to higher values for the ratio carotenoids/chlorophylls. Finally, the effects of pressure and temperature on the extraction yields of the supercritical fluid extraction process were studied.
在本文所述的工作中，使用两种提取技术，即超声辅助提取和超临界流体提取，对类胡萝卜素和叶绿素的提取过程进行了分析，并对结果进行了比较。用于超声辅助提取的溶剂是N，N'-二甲基甲酰胺和甲醇，用于超临界流体提取的溶剂是二氧化碳。所研究的原料是盐生杜氏藻，这是一种微藻，其细胞结构中含有高水平的类胡萝卜素。结果表明，当使用甲醇作为溶剂时，超临界流体提取过程与超声辅助提取相当。此外，超临界提取过程在类胡萝卜素回收方面比传统技术更具选择性，因为它导致类胡萝卜素/叶绿素的比例更高。最后，研究了压力和温度对超临界流体提取过程提取产率的影响。
