Natural Products Research Center, Korea Institute of Science and Technology, Gangneung, Gangwon-do 210-340, Korea.
J Agric Food Chem. 2010 Jan 27;58(2):793-7. doi: 10.1021/jf902628j.
Pressurized liquid extraction (PLE) was applied to the extraction of carotenoids and chlorophylls from the green microalga Chlorella vulgaris. Four extraction techniques such as maceration (MAC), Soxhlet extraction (SOX), ultrasound assisted extraction (UAE), and PLE were compared, and both the extraction temperature (50, 105, and 160 degrees C) and the extraction time (8, 19, and 30 min), which are the two main factors for PLE, were optimized with a central composite design to obtain the highest extraction efficiency. The extraction solvent (90% ethanol/water) could adequately extract the functional components from C. vulgaris. PLE showed higher extraction efficiencies than MAC, SOX, and UAE. Temperature was the key parameter having the strongest influence on the extraction of carotenoids and chlorophylls from chlorella. In addition, high heat treatment (>110 degrees C) by PLE minimized the formation of pheophorbide a, a harmful chlorophyll derivative. These results indicate that PLE may be a useful extraction method for the simultaneous extraction of carotenoids and chlorophylls from C. vulgaris.
加压液体萃取（PLE）被应用于从绿藻小球藻中提取类胡萝卜素和叶绿素。比较了四种萃取技术，包括浸泡（MAC）、索氏提取（SOX）、超声辅助提取（UAE）和 PLE，并通过中心复合设计优化了萃取温度（50、105 和 160°C）和萃取时间（8、19 和 30 分钟），以获得最高的萃取效率。萃取溶剂（90%乙醇/水）可以从小球藻中充分提取功能性成分。PLE 的萃取效率高于 MAC、SOX 和 UAE。温度是对小球藻中类胡萝卜素和叶绿素提取影响最强的关键参数。此外，PLE 的高温处理（>110°C）最大限度地减少了有害的叶绿素衍生物脱镁叶绿酸 a 的形成。这些结果表明，PLE 可能是从小球藻中同时提取类胡萝卜素和叶绿素的一种有用的提取方法。
