Department of Obstetrics and Gynecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119074.
Fertil Steril. 2010 Feb;93(3):986-94. doi: 10.1016/j.fertnstert.2008.10.030. Epub 2008 Dec 6.
To explore several culture systems that may prove efficient in driving human embryonic stem cells (hESCs) toward a germ cell lineage.
Embryoid bodies (EBs) derived from a female hESC line [HES-3 (XX)] and male hESC line [HES-4 (XY)] were cultured in six different culture conditions: [1] mitotically inactivated porcine ovarian fibroblasts (POF), [2] 100% conditioned medium from POF, [3] 50% conditioned medium from POF, [4] forskolin, [5] trans-retinoic acid (RA), and [6] forskolin and RA.
Department of Obstetrics and Gynecology, National University of Singapore Research Laboratories, Singapore.
PATIENT(S): None.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): None.
RESULT(S): Expression data for both HES-3 and HES-4 differentiating cultures strongly indicated that inactivated POFs encouraged differentiation of hESC EBs into a germ cell lineage. VASA and other germ cell markers were found to be elevated in all six culture conditions.
CONCLUSION(S): Overall, POFs proved to be the best system for initiating germ cell differentiation, as shown by increases in the expression of several germ cell marker genes in EBs that were cocultured with POFs.
探索几种可能有效促使人类胚胎干细胞（hESC）向生殖细胞系分化的培养体系。
从女性 hESC 系 [HES-3（XX）] 和男性 hESC 系 [HES-4（XY）] 中获得类胚体（EB），在 6 种不同的培养条件下进行培养：[1] 有丝分裂失活的猪卵巢成纤维细胞（POF），[2] 来自 POF 的 100%条件培养基，[3] 来自 POF 的 50%条件培养基，[4] forskolin，[5] 视黄酸（RA），和[6] forskolin 和 RA。
新加坡国立大学妇产科系，新加坡国立大学研究实验室。
患者（s）：无。
干预（s）：无。
主要观察指标（s）：无。
结果（s）：HES-3 和 HES-4 分化培养的表达数据强烈表明，失活的 POF 促进了 hESC EB 向生殖细胞系分化。在所有 6 种培养条件下均发现 VASA 和其他生殖细胞标记物的表达升高。
结论（s）：总之，POF 被证明是启动生殖细胞分化的最佳系统，因为与 POF 共培养的 EB 中几个生殖细胞标记基因的表达增加证明了这一点。
