Romanos G, Schröter-Kermani C, Hinz N, Bernimoulin J P
Department of Periodontology, Dental School North, Freie Universität Berlin, FRG.
Matrix. 1991 Apr;11(2):125-32. doi: 10.1016/s0934-8832(11)80216-7.
The purpose of this study was to demonstrate the localization of collagen types IV, V and VI as well as the glycoprotein laminin in biopsies of healthy rat, marmoset (Callithrix jacchus) and human gingivae. The slices, after the use of indirect immunofluorescence (incubation with antibodies against these extracellular matrix components), showed the same distribution with the anti-type IV and laminin antibodies on the basement membranes of the epithelium, blood vessels and nerves. Collagen type V, as a filamentous, and collagen type VI, as microfibrillar components, were localized in a similar pattern in the different species. In contrast to the other species, collagen type VI could not be found near the basement membranes of vessels and nerves of the marmoset gingiva. This result shows differences between human and monkey tissues, but not between rat and human gingivae, and conforms the heterogeneity of collagen type VI in the various cell and tissue types.
本研究的目的是在健康大鼠、狨猴（绢毛猴）和人类牙龈组织活检样本中，展示IV型、V型和VI型胶原蛋白以及糖蛋白层粘连蛋白的定位情况。切片在采用间接免疫荧光法（与针对这些细胞外基质成分的抗体孵育）后，使用抗IV型抗体和层粘连蛋白抗体观察到上皮、血管和神经基底膜上呈现相同的分布情况。V型胶原蛋白以丝状形式存在，VI型胶原蛋白以微纤维成分形式存在，在不同物种中呈现相似的定位模式。与其他物种不同，在狨猴牙龈的血管和神经基底膜附近未发现VI型胶原蛋白。这一结果显示了人类组织与猴组织之间的差异，但大鼠和人类牙龈组织之间未发现差异，这也证实了VI型胶原蛋白在不同细胞和组织类型中的异质性。
