Zhu Qi, Wang Jing, Lai Hua-mei
Department of Gastroenterology, Ruijin Hospital Affiliated to Medical College of Shanghai Jiaotong University, Shanghai.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2008 Sep;28(9):813-7.
To explore the mechanism and efficiency of Changji'an (CJA) in treating irritable bowel syndrome through studying the relationship between serotonin transporter (SERT) and visceral hypersensitivity in rats.
Male SD rats were randomly divided into 4 groups: the normal control group, the model group, the high-dosage and low-dosage CJA (CJAH and CJAL) groups. Visceral hypersensitivity model was established by colorectal distension. Normal saline and different doses of CJA were administrated to rats respectively, starting from the 10th day of modeling for 10 days. After then, the abdominal withdrawal reflex (AWR) was scored for semi-quantitative estimation of visceral sensitivity, and tissues of brain and colon were harvested for detecting expressions of SERT and serotonin (5-HT) with Western blot, real-time PCR and immunohistochemistry.
As compared with the normal controls, in model rats, the AWR score and content of 5-HT in intestinal mucosa were higher (P < 0.05), protein and mRNA expressions of SERT in colon and nucleus raphes dorsalis (NRD) were lower (P < 0.05), but all these indexes were improved significantly after CJA treatment, either in the CJAH or CJAL group (all P < 0.05). Besides, the number of 5-HT energic neuron in the model group and CJA groups was lower than that in the normal control group (P < 0.05).
CJA has therapeutic effect for improving visceral hypersensitivity in irritable bowel syndrome by way of regulating colonic expression of SERT and content of 5-HT.
通过研究血清素转运体（SERT）与大鼠内脏高敏感性之间的关系，探讨肠激安（CJA）治疗肠易激综合征的机制和疗效。
雄性SD大鼠随机分为4组：正常对照组、模型组、肠激安高剂量组和低剂量组（CJAH和CJAL）。采用结直肠扩张法建立内脏高敏感性模型。从造模第10天开始，分别给大鼠灌胃生理盐水和不同剂量的肠激安，持续10天。之后，对腹部退缩反射（AWR）进行评分，以半定量评估内脏敏感性，并采集脑和结肠组织，用蛋白质免疫印迹法、实时荧光定量PCR和免疫组织化学法检测SERT和血清素（5-HT）的表达。
与正常对照组相比，模型大鼠的AWR评分和肠黏膜5-HT含量升高（P<0.05），结肠和中缝背核（NRD）中SERT的蛋白和mRNA表达降低（P<0.05），但经肠激安治疗后，CJAH组和CJAL组的上述指标均显著改善（均P<0.05）。此外，模型组和肠激安组5-HT能神经元数量低于正常对照组（P<0.05）。
肠激安可通过调节结肠SERT表达和5-HT含量来改善肠易激综合征的内脏高敏感性。
